Interleukin-10 inhibits spontaneous sleep in rabbits

Tetsuya Kushikata, Jidong Fang, James M. Krueger

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


Proinflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) are involved in sleep regulation. IL-10 is an anti-inflammatory cytokine that inhibits proinflammatory cytokine production. We hypothesized that IL-10 could attenuate sleep. Thirty-one male rabbits were used. Three doses of IL-10 (5 ng, 50 ng, and 250 ng) were injected intracerebroventricularly during the rest (light) period. One dose of IL-10 (250 ng) was injected during the active (dark) cycle. Appropriate time- matched control injections of saline were given to the same rabbits on different days. The two highest doses of IL-10 significantly inhibited spontaneous nonrapid eye movement sleep if IL-10 was given during the light cycle. The highest dose of IL-10 (250 ng) also significantly decreased rapid eye movement sleep. IL-10 administered at dark onset had no effect on sleep. The sleep inhibitory properties of IL-10 provide additional evidence for the hypothesis that a brain cytokine network is involved in regulation of physiologic sleep.

Original languageEnglish (US)
Pages (from-to)1025-1030
Number of pages6
JournalJournal of Interferon and Cytokine Research
Issue number9
StatePublished - 1999

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology


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