TY - JOUR
T1 - Intracellular binding proteins for retinol and retinoic acid
T2 - Comparison with each other and with serum retinol-binding protein
AU - Ross, A. Catharine
AU - Goodman, D. S.
PY - 1979
Y1 - 1979
N2 - Soluble proteins with binding specificity for either retinol or retinoic acid were isolated from rat testis cytosol. The characteristics of the purified cytosolic proteins were compared both with each other, and directly with those of serum retinol-binding protein (RBP). substantial similarities were observed between the two cytosol retinoid binding proteins, as wells as clear differences between each of these proteins and serum RBP. The intracellular binding proteins differed from serum RBP with regard to molecular weight, immuno-reactivity, binding affinity for serum prealbumin, and ultraviolet and fluorescence spectral characteristics. The ultraviolet absorption spectrums of the cytosol binding protein for retinol and of the cytosol binding protein for retinoic acid were almost identical despite differences in the nature of the ligands bound by these two proteins.
AB - Soluble proteins with binding specificity for either retinol or retinoic acid were isolated from rat testis cytosol. The characteristics of the purified cytosolic proteins were compared both with each other, and directly with those of serum retinol-binding protein (RBP). substantial similarities were observed between the two cytosol retinoid binding proteins, as wells as clear differences between each of these proteins and serum RBP. The intracellular binding proteins differed from serum RBP with regard to molecular weight, immuno-reactivity, binding affinity for serum prealbumin, and ultraviolet and fluorescence spectral characteristics. The ultraviolet absorption spectrums of the cytosol binding protein for retinol and of the cytosol binding protein for retinoic acid were almost identical despite differences in the nature of the ligands bound by these two proteins.
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M3 - Article
C2 - 573704
AN - SCOPUS:0018691973
SN - 0014-9446
VL - 38
SP - 2515
EP - 2518
JO - Federation Proceedings
JF - Federation Proceedings
IS - 11
ER -