TY - JOUR
T1 - Intrapreoptic microinjection of GHRH or its antagonist alters sleep in rats
AU - Zhang, Jianyi
AU - Obál, Ferenc
AU - Zheng, Tong
AU - Fang, Jidong
AU - Taishi, Ping
AU - Krueger, James M.
N1 - Funding Information:
We particularly thank the family members for accepting the invitation to participate in the present research study. We also thank Annie Roy, Mireille Poulin, Carole Lehouillier, and Yah Théberge for technical assistance in immortalization of cells and genotyping, psychiatric nurses Janine Dussault and Jocelyne Dallaire for interviewing the subjects, Marie-Josée Lord for her participation in the clinical evaluation, and Marc De Braekeleer for his precious knowledge of the Charlevoix region. Special thanks are due to Mr. Harold Gaboury for his professional help in editing the manuscript in English. This research was supported by Medical Research Council of Canada grant MT-12091 (to C.C.), les Fonds de la recherche en santé du Québec research scholarships 940270-103 (to C.C.) and 970047 (to C.M.), and U.S. National Institute of Mental Health grant MH44292 (to J.O.).
PY - 1999/3/15
Y1 - 1999/3/15
N2 - Previous reports indicate that growth hormone-releasing hormone (GHRH) is involved in sleep regulation. The site of action mediating the nonrapid eye movement sleep (NREMS)-promoting effects of GHRH is not known, but it is independent from the pituitary. GHRH (0.001, 0.01, and 0.1 nmol/kg) or a competitive antagonist of GHRH (0.003, 0.3, and 14 nmol/kg) was microinjected into the preoptic area, and the sleep-wake activity was recorded for 23 hr after injection in rats. GHRH elicited dose-dependent increases in the duration and in the intensity of NREMS compared with that in control records after intrapreoptic injection of physiological saline. The antagonist decreased the duration and intensity of NREMS and prolonged sleep latency. Consistent alterations in rapid eye movement sleep (REMS) and in brain temperature were not found. The GHRH antagonist also attenuated the enhancements in NREMS elicited by 3 hr of sleep deprivation. Histological verification of the injection sites showed that the majority of the effective injections were in the preoptic area and the diagonal band of Broca. The results indicate that the preoptic area mediates the sleep-promoting activity of GHRH.
AB - Previous reports indicate that growth hormone-releasing hormone (GHRH) is involved in sleep regulation. The site of action mediating the nonrapid eye movement sleep (NREMS)-promoting effects of GHRH is not known, but it is independent from the pituitary. GHRH (0.001, 0.01, and 0.1 nmol/kg) or a competitive antagonist of GHRH (0.003, 0.3, and 14 nmol/kg) was microinjected into the preoptic area, and the sleep-wake activity was recorded for 23 hr after injection in rats. GHRH elicited dose-dependent increases in the duration and in the intensity of NREMS compared with that in control records after intrapreoptic injection of physiological saline. The antagonist decreased the duration and intensity of NREMS and prolonged sleep latency. Consistent alterations in rapid eye movement sleep (REMS) and in brain temperature were not found. The GHRH antagonist also attenuated the enhancements in NREMS elicited by 3 hr of sleep deprivation. Histological verification of the injection sites showed that the majority of the effective injections were in the preoptic area and the diagonal band of Broca. The results indicate that the preoptic area mediates the sleep-promoting activity of GHRH.
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U2 - 10.1523/jneurosci.19-06-02187.1999
DO - 10.1523/jneurosci.19-06-02187.1999
M3 - Article
C2 - 10066272
AN - SCOPUS:0033559845
SN - 0270-6474
VL - 19
SP - 2187
EP - 2194
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 6
ER -