TY - JOUR
T1 - Intratumoral generation of photothermal gold nanoparticles through a vectorized biomineralization of ionic gold
AU - Schwartz-Duval, Aaron S.
AU - Konopka, Christian J.
AU - Moitra, Parikshit
AU - Daza, Enrique A.
AU - Srivastava, Indrajit
AU - Johnson, Elyse V.
AU - Kampert, Taylor L.
AU - Fayn, Stanley
AU - Haran, Anand
AU - Dobrucki, Lawrence W.
AU - Pan, Dipanjan
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Various cancer cells have been demonstrated to have the capacity to form plasmonic gold nanoparticles when chloroauric acid is introduced to their cellular microenvironment. But their biomedical applications are limited, particularly considering the millimolar concentrations and longer incubation period of ionic gold. Here, we describe a simplistic method of intracellular biomineralization to produce plasmonic gold nanoparticles at micromolar concentrations within 30 min of application utilizing polyethylene glycol as delivery vector for ionic gold. We have characterized this process for intracellular gold nanoparticle formation, which progressively accumulates proteins as the ionic gold clusters migrate to the nucleus. This nano-vectorized application of ionic gold emphasizes its potential biomedical opportunities while reducing the quantity of ionic gold and required incubation time. To demonstrate its biomedical potential, we further induce in-situ biosynthesis of gold nanoparticles within MCF7 tumor mouse xenografts which is followed by its photothermal remediation.
AB - Various cancer cells have been demonstrated to have the capacity to form plasmonic gold nanoparticles when chloroauric acid is introduced to their cellular microenvironment. But their biomedical applications are limited, particularly considering the millimolar concentrations and longer incubation period of ionic gold. Here, we describe a simplistic method of intracellular biomineralization to produce plasmonic gold nanoparticles at micromolar concentrations within 30 min of application utilizing polyethylene glycol as delivery vector for ionic gold. We have characterized this process for intracellular gold nanoparticle formation, which progressively accumulates proteins as the ionic gold clusters migrate to the nucleus. This nano-vectorized application of ionic gold emphasizes its potential biomedical opportunities while reducing the quantity of ionic gold and required incubation time. To demonstrate its biomedical potential, we further induce in-situ biosynthesis of gold nanoparticles within MCF7 tumor mouse xenografts which is followed by its photothermal remediation.
UR - http://www.scopus.com/inward/record.url?scp=85090501468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85090501468&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-17595-6
DO - 10.1038/s41467-020-17595-6
M3 - Article
C2 - 32913195
AN - SCOPUS:85090501468
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 4530
ER -
