Intrinsic focal variations of rabbit aortic hydrolase activities

Ronald Albert Markle

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Normal arterial foci which take up Evans blue dye (EBD) in vivo are believed to represent atherosclerosis-prone, hemodynamically stressed foci compared to areas which exclude dye. We have used the rabbit EBD model to examine focal aortic hydrolases of blue areasa versus white areas, and we report herein significant focal variations of hydrolase activities. Enzymes measured included neutral α-glucosidase, N-acetyl-β-glucosaminidase, α-mannosidase, acid α-glucosidase, β-galactosidase, β-glucuronidase, cathepsin C, and acid cholesteryl esterase (ACE); specific activities were expressed on the basis of tissue DNA. In correlative areas of EBD uptake in normal rabbit aortic arch, ACE activity averaged 17% higher and cathepsin C activity averaged 37% lower than activities of areas free of EBD in the descending thoracic aorta (P < 0.02). None of the glycosidases studied differed significantly between blue and white aortic areas. These findings indicate that discrete, intrinsic differences of hydrolytic enzyme activities exist in the normal rabbit aorta in areas delineated by in vivo EBD uptake, areas recognized as lesion-prone vs lesion-resistant.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalExperimental and Molecular Pathology
Issue number1
StatePublished - Jan 1 1989

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine


Dive into the research topics of 'Intrinsic focal variations of rabbit aortic hydrolase activities'. Together they form a unique fingerprint.

Cite this