Invasion of Solanum tuberosum L. by Aspergillus terreus: A microscopic and proteomics insight on pathogenicity

Bengyella Louis, Sayanika Devi Waikhom, Pranab Roy, Pardeep Kumar Bhardwaj, Mohendro Wakambam Singh, Sharma K. Chandradev, Narayan Chandra Talukdar

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Background: Aspergillus terreus is one of the most harmful filamentous fungal pathogen of humans, animals and plants. Recently, researchers have discovered that A. terreus can cause foliar blight disease in potato (Solanum tuberosum L.). We used light and scanning electron microscopy, and performed proteomics analysis in an attempt to dissect the invasion process of A. terreus in this important crop. Results: Microscopic study revealed that invasion of leaf tissue is marked by rapid germination of A. terreus phialidic conidia (PC) by 4 h after inoculation. By 8 h after inoculation, primary germ tubes from PC differentiated into irregular protuberance, often displayed stomata atropism, and failed to penetrate via the epidermal cells. Colonization of leaf tissues was associated with high rate of production of accessory conidia (AC). These analyses showed the occurrence of a unique opposing pattern of AC, tissue-specific and produced on melanized colonizing hyphae during the infection of leaf tissue. A significant proteome change hallmarked by differential expression of class I patatin, lipoxygenase, catalase-peroxidase complex, and cysteine proteinase inhibitor were observed during tuber colonization. These proteins are often involved in signal transduction pathways and crosstalk in pathogenic responses. Conclusion: A. terreus abundantly produced AC and multipolar germinating PC to invade potato leaf tissue. Additionally, A. terreus differentially induced enzymes in potato tuber during colonization which facilitates rapid disease development.

Original languageEnglish (US)
Article number350
JournalBMC Research Notes
Volume7
Issue number1
DOIs
StatePublished - Jun 10 2014

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology

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