TY - JOUR
T1 - Invasion of Solanum tuberosum L. by Aspergillus terreus
T2 - A microscopic and proteomics insight on pathogenicity
AU - Louis, Bengyella
AU - Waikhom, Sayanika Devi
AU - Roy, Pranab
AU - Bhardwaj, Pardeep Kumar
AU - Singh, Mohendro Wakambam
AU - Chandradev, Sharma K.
AU - Talukdar, Narayan Chandra
N1 - Funding Information:
This research was jointly supported by the Academy of Sciences for Developing World (TWAS), Trieste, Italy and the Department of Biotechnology, Government of India (Program No. 3240223450). The authors thank DK Hore, RC Rashmi and N Mazumder for proofreading the manuscript. The University Science Instrumentation Centre, Burdwan University for some of the SEM micrographs.
PY - 2014/6/10
Y1 - 2014/6/10
N2 - Background: Aspergillus terreus is one of the most harmful filamentous fungal pathogen of humans, animals and plants. Recently, researchers have discovered that A. terreus can cause foliar blight disease in potato (Solanum tuberosum L.). We used light and scanning electron microscopy, and performed proteomics analysis in an attempt to dissect the invasion process of A. terreus in this important crop. Results: Microscopic study revealed that invasion of leaf tissue is marked by rapid germination of A. terreus phialidic conidia (PC) by 4 h after inoculation. By 8 h after inoculation, primary germ tubes from PC differentiated into irregular protuberance, often displayed stomata atropism, and failed to penetrate via the epidermal cells. Colonization of leaf tissues was associated with high rate of production of accessory conidia (AC). These analyses showed the occurrence of a unique opposing pattern of AC, tissue-specific and produced on melanized colonizing hyphae during the infection of leaf tissue. A significant proteome change hallmarked by differential expression of class I patatin, lipoxygenase, catalase-peroxidase complex, and cysteine proteinase inhibitor were observed during tuber colonization. These proteins are often involved in signal transduction pathways and crosstalk in pathogenic responses. Conclusion: A. terreus abundantly produced AC and multipolar germinating PC to invade potato leaf tissue. Additionally, A. terreus differentially induced enzymes in potato tuber during colonization which facilitates rapid disease development.
AB - Background: Aspergillus terreus is one of the most harmful filamentous fungal pathogen of humans, animals and plants. Recently, researchers have discovered that A. terreus can cause foliar blight disease in potato (Solanum tuberosum L.). We used light and scanning electron microscopy, and performed proteomics analysis in an attempt to dissect the invasion process of A. terreus in this important crop. Results: Microscopic study revealed that invasion of leaf tissue is marked by rapid germination of A. terreus phialidic conidia (PC) by 4 h after inoculation. By 8 h after inoculation, primary germ tubes from PC differentiated into irregular protuberance, often displayed stomata atropism, and failed to penetrate via the epidermal cells. Colonization of leaf tissues was associated with high rate of production of accessory conidia (AC). These analyses showed the occurrence of a unique opposing pattern of AC, tissue-specific and produced on melanized colonizing hyphae during the infection of leaf tissue. A significant proteome change hallmarked by differential expression of class I patatin, lipoxygenase, catalase-peroxidase complex, and cysteine proteinase inhibitor were observed during tuber colonization. These proteins are often involved in signal transduction pathways and crosstalk in pathogenic responses. Conclusion: A. terreus abundantly produced AC and multipolar germinating PC to invade potato leaf tissue. Additionally, A. terreus differentially induced enzymes in potato tuber during colonization which facilitates rapid disease development.
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U2 - 10.1186/1756-0500-7-350
DO - 10.1186/1756-0500-7-350
M3 - Article
C2 - 24917207
AN - SCOPUS:84903310826
SN - 1756-0500
VL - 7
JO - BMC Research Notes
JF - BMC Research Notes
IS - 1
M1 - 350
ER -