@article{ddce5a6e860e43929a34e7ff7c0d4875,
title = "Investigating pioneer factor activity and its coordination with chromatin remodelers using integrated synthetic oligo assay",
abstract = "Chromatin accessibility is regulated by pioneer factors (PFs) and chromatin remodelers (CRs). Here, we present a protocol, based on integrated synthetic oligonucleotide libraries in yeast, to systematically interrogate the nucleosome-displacing activities of PFs and their coordination with CRs. We describe steps for designing oligonucleotide sequences, constructing yeast libraries, measuring nucleosome configurations, and data analyses. This approach potentially can be adapted for use in higher eukaryotes to investigate the activities of many types of chromatin-associated factors. For complete details on the use and execution of this protocol, please refer to Yan et al.,1 and Chen et al.2",
author = "Hengye Chen and Chao Yan and Archana Dhasarathy and Michael Kladde and Lu Bai",
note = "Funding Information: We thank the members of Bai Lab and the Center of Eukaryotic Gene Regulation at PSU for discussions and technical support. This work is supported by the National Institutes of Health (R35 GM139654 to L.B. and R01 CA095525 and R01 CA155390 to M.P.K.). L.B. H.C. and C.Y. designed the experiments and performed data analysis; H.C. and C.Y. performed the experiments; A.D. and M.K. constructed the DNMT expression systems; H.C. and L.B. wrote the manuscript. The authors declare no conflict of interest. Funding Information: We thank the members of Bai Lab and the Center of Eukaryotic Gene Regulation at PSU for discussions and technical support. This work is supported by the National Institutes of Health ( R35 GM139654 to L.B. and R01 CA095525 and R01 CA155390 to M.P.K.). Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = jun,
day = "16",
doi = "10.1016/j.xpro.2023.102279",
language = "English (US)",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}