Investigation of an antibody-ligase. Evidence for strain-induced catalysis

Sergey N. Savinov; Ralph Hirschmann; Stephen J. Benkovic; Amos B. Smith

doi:10.1016/S0960-894X(03)00123-9

Investigation of an antibody-ligase. Evidence for strain-induced catalysis

Sergey N. Savinov, Ralph Hirschmann, Stephen J. Benkovic, Amos B. Smith

Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Catalytic antibody 16G3, a peptide ligase with extended substrate scope has been characterized mechanistically exploiting a set of systematically designed perspective substrates 6-9, two of which, thioesters 8 and 9 act instead as inhibitors. Taken together the structure/activity relationships suggest a catalytic mechanism dependent on induction of strain, programmed via specific structural deviations between the hapten and the substrates. General mechanistic implications for de novo induced catalysis are presented.

Original language	English (US)
Pages (from-to)	1321-1324
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	13
Issue number	7
DOIs	https://doi.org/10.1016/S0960-894X(03)00123-9
State	Published - Apr 7 2003

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/S0960-894X(03)00123-9

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AU - Hirschmann, Ralph

AU - Benkovic, Stephen J.

AU - Smith, Amos B.

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AB - Catalytic antibody 16G3, a peptide ligase with extended substrate scope has been characterized mechanistically exploiting a set of systematically designed perspective substrates 6-9, two of which, thioesters 8 and 9 act instead as inhibitors. Taken together the structure/activity relationships suggest a catalytic mechanism dependent on induction of strain, programmed via specific structural deviations between the hapten and the substrates. General mechanistic implications for de novo induced catalysis are presented.

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Investigation of an antibody-ligase. Evidence for strain-induced catalysis

Abstract

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