Investigation of an antibody-ligase. Evidence for strain-induced catalysis

Sergey N. Savinov; Ralph Hirschmann; Stephen J. Benkovic; Amos B. Smith

doi:10.1016/S0960-894X(03)00123-9

Investigation of an antibody-ligase. Evidence for strain-induced catalysis

Sergey N. Savinov
, Ralph Hirschmann
, Stephen J. Benkovic
, Amos B. Smith

Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Catalytic antibody 16G3, a peptide ligase with extended substrate scope has been characterized mechanistically exploiting a set of systematically designed perspective substrates 6-9, two of which, thioesters 8 and 9 act instead as inhibitors. Taken together the structure/activity relationships suggest a catalytic mechanism dependent on induction of strain, programmed via specific structural deviations between the hapten and the substrates. General mechanistic implications for de novo induced catalysis are presented.

Original language	English (US)
Pages (from-to)	1321-1324
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	13
Issue number	7
DOIs	https://doi.org/10.1016/S0960-894X(03)00123-9
State	Published - Apr 7 2003

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/S0960-894X(03)00123-9

Cite this

@article{77d63cc17fd74dcf92f8718851cf853e,

title = "Investigation of an antibody-ligase. Evidence for strain-induced catalysis",

abstract = "Catalytic antibody 16G3, a peptide ligase with extended substrate scope has been characterized mechanistically exploiting a set of systematically designed perspective substrates 6-9, two of which, thioesters 8 and 9 act instead as inhibitors. Taken together the structure/activity relationships suggest a catalytic mechanism dependent on induction of strain, programmed via specific structural deviations between the hapten and the substrates. General mechanistic implications for de novo induced catalysis are presented.",

author = "Savinov, \{Sergey N.\} and Ralph Hirschmann and Benkovic, \{Stephen J.\} and Smith, \{Amos B.\}",

note = "Funding Information: This work was supported by a grant from the National Institute of Health (GM 45611). ",

year = "2003",

month = apr,

day = "7",

doi = "10.1016/S0960-894X(03)00123-9",

language = "English (US)",

volume = "13",

pages = "1321--1324",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

number = "7",

}

TY - JOUR

T1 - Investigation of an antibody-ligase. Evidence for strain-induced catalysis

AU - Savinov, Sergey N.

AU - Hirschmann, Ralph

AU - Benkovic, Stephen J.

AU - Smith, Amos B.

N1 - Funding Information: This work was supported by a grant from the National Institute of Health (GM 45611).

PY - 2003/4/7

Y1 - 2003/4/7

N2 - Catalytic antibody 16G3, a peptide ligase with extended substrate scope has been characterized mechanistically exploiting a set of systematically designed perspective substrates 6-9, two of which, thioesters 8 and 9 act instead as inhibitors. Taken together the structure/activity relationships suggest a catalytic mechanism dependent on induction of strain, programmed via specific structural deviations between the hapten and the substrates. General mechanistic implications for de novo induced catalysis are presented.

AB - Catalytic antibody 16G3, a peptide ligase with extended substrate scope has been characterized mechanistically exploiting a set of systematically designed perspective substrates 6-9, two of which, thioesters 8 and 9 act instead as inhibitors. Taken together the structure/activity relationships suggest a catalytic mechanism dependent on induction of strain, programmed via specific structural deviations between the hapten and the substrates. General mechanistic implications for de novo induced catalysis are presented.

UR - https://www.scopus.com/pages/publications/0037424712

UR - https://www.scopus.com/pages/publications/0037424712#tab=citedBy

U2 - 10.1016/S0960-894X(03)00123-9

DO - 10.1016/S0960-894X(03)00123-9

M3 - Article

C2 - 12657273

AN - SCOPUS:0037424712

SN - 0960-894X

VL - 13

SP - 1321

EP - 1324

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 7

ER -

Investigation of an antibody-ligase. Evidence for strain-induced catalysis

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this