Iodination of plasma membrane proteins of BHK cells in different growth states

Andrea M. Mastro; Charles T. Beer; Gerald C. Mueller

doi:10.1016/0005-2736(74)90177-1

Iodination of plasma membrane proteins of BHK cells in different growth states

Andrea M. Mastro, Charles T. Beer, Gerald C. Mueller

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with ¹²⁵I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.

Original language	English (US)
Pages (from-to)	38-51
Number of pages	14
Journal	BBA - Biomembranes
Volume	352
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(74)90177-1
State	Published - May 30 1974

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(74)90177-1

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title = "Iodination of plasma membrane proteins of BHK cells in different growth states",

abstract = "The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.",

author = "Mastro, {Andrea M.} and Beer, {Charles T.} and Mueller, {Gerald C.}",

note = "Funding Information: The study was supported by a U.S. Public Health Service Grant TO1-CA-5002 and U.S. Public Health Service Grant CA-07175. A. M. M. was a Damon Runyon Postdoctoral Fellow; C. T. B., a visiting Professor from the Cancer Research Centre, University of British Columbia, was supported by the Medical Research Council of Canada; and G. C. M. is the recipient of a research career award, U.S. Public Health Service. The authors thank Ms Mary Le Mahieu for her help in the preparation of this manuscript.",

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doi = "10.1016/0005-2736(74)90177-1",

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T1 - Iodination of plasma membrane proteins of BHK cells in different growth states

AU - Mastro, Andrea M.

AU - Beer, Charles T.

AU - Mueller, Gerald C.

N1 - Funding Information: The study was supported by a U.S. Public Health Service Grant TO1-CA-5002 and U.S. Public Health Service Grant CA-07175. A. M. M. was a Damon Runyon Postdoctoral Fellow; C. T. B., a visiting Professor from the Cancer Research Centre, University of British Columbia, was supported by the Medical Research Council of Canada; and G. C. M. is the recipient of a research career award, U.S. Public Health Service. The authors thank Ms Mary Le Mahieu for her help in the preparation of this manuscript.

PY - 1974/5/30

Y1 - 1974/5/30

N2 - The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.

AB - The surfaces of BHK cells in confluent monolayers, immediately after mechanical dispersal and in logarithmically growing suspension cultures have been iodinated with 125I using the lactoperoxidase technique. Electrophoretic resolution of the labeled proteins revealed that the representation of plasma membrane proteins varies with the growth state. Trypsinization of the cells produced a drastic revision of the surfaces leaving behind root fragments of membrane components and exposing additional proteins for iodination. The rapid turnover of membrane proteins in growing BHK cells restored the plasma membrane to a state characteristic of the replicating cell within 10 h.

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JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 1

ER -

Iodination of plasma membrane proteins of BHK cells in different growth states

Abstract

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