Abstract
Polyanionic oligosaccharides such as dermatan sulfate (DS) and chondroitin sulfate (CS) exhibit poor ionization efficiencies and tend to undergo thermal fragmentation through the loss of SO3 under conventional ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI) conditions. A new ionic liquid matrix (ILM), a guanidinium salt of α-cyano-4-hydroxycinnamic acid, facilitates direct UV-MALDI mass spectrometric (MS) analysis of underivatized DS and CS oligosaccharides up to a decasaccharide in their common form as sodium salts. The resulting mass spectra show very low extent of fragmentation through an SO3 loss. The new ILM is suitable for MALDI-MS analysis of mixtures containing oligosaccharides with different numbers of sulfo groups.
Original language | English (US) |
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Pages (from-to) | 1604-1610 |
Number of pages | 7 |
Journal | Analytical Chemistry |
Volume | 79 |
Issue number | 4 |
DOIs | |
State | Published - Feb 15 2007 |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry