Iron regulates expression of Bacillus cereus Hemolysin II via global regulator Fur

Elena Sineva, Andrey Shadrin, Ekaterina A. Rodikova, Zhanna I. Andreeva-Kovalevskaya, Alexey S. Protsenko, Sergey G. Mayorov, Darya Yu Galaktionova, Erica Magelky, Alexander S. Solonin

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


The capacity of pathogens to respond to environmental signals, such as iron concentration, is key to bacterial survival and establishment of a successful infection. Bacillus cereus is a widely distributed bacterium with distinct pathogenic properties. Hemolysin II (HlyII) is one of its pore-forming cytotoxins and has been shown to be involved in bacterial pathogenicity in a number of cell and animal models. Unlike many other B. cereus pathogenicity factors, HlyII is not regulated by pleiotropic transcriptional regulator PlcR but is controlled by its own regulator, HlyIIR. Using a combination of in vivo and in vitro techniques, we show that hlyII expression is also negatively regulated by iron by the global regulator Fur via direct interaction with the hlyII promoter. DNase I footprinting and in vitro transcription experiments indicate that Fur prevents RNA polymerase binding to the hlyII promoter. HlyII expression profiles demonstrate that both HlyIIR and Fur regulate HlyII expression in a concerted fashion, with the effect of Fur being maximal in the early stages of bacterial growth. In sum, these results show that Fur serves as a transcriptional repressor for hlyII expression.

Original languageEnglish (US)
Pages (from-to)3327-3335
Number of pages9
JournalJournal of bacteriology
Issue number13
StatePublished - Jul 2012

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology


Dive into the research topics of 'Iron regulates expression of Bacillus cereus Hemolysin II via global regulator Fur'. Together they form a unique fingerprint.

Cite this