Abstract
We report the first successful isolation and initial characterization of S-adenosylmethionine decarboxylase (SAMDC)-overexpressing cells using a transfection approach. Stably transfected MCF-7 breast cancer cells overproducing SAMDC (~5-fold) manifested reduced ornithine decarboxylase while levels of N′-spermidine/spermine acetyltransferase were variably increased. Analysis of cellular polyamine profile showed that spermine was selectively increased (~80%), while spermidine and putrescine levels were reduced (~48% and ~15% of control, respectively). Since SAMDC-overexpressing clones exhibited increased clonogenicity in soft agar, our data suggest that spermine may be selectively involved in conferring a more invasive phenotype to breast cancer cells.
Original language | English (US) |
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Pages (from-to) | 23-28 |
Number of pages | 6 |
Journal | Cancer Letters |
Volume | 95 |
Issue number | 1-2 |
DOIs | |
State | Published - Aug 16 1995 |
All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research