Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides

T. L. King; M. S. Eackles; B. A. Garner; M. van Tuinen; B. S. Arbogast

doi:10.1007/s12686-015-0456-0

Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides

T. L. King, M. S. Eackles, B. A. Garner, M. van Tuinen, B. S. Arbogast

Ecosystem Science and Management

Research output: Contribution to journal › Letter › peer-review

Abstract

The hermaphroditic flat-spired three-tooth land snail (Triodopsis platysayoides) is endemic to a 21-km stretch of the Cheat River Gorge of northeastern West Virginia, USA. We document isolation and characterization of ten microsatellite DNA markers in this at-risk species. The markers displayed a moderate level of allelic diversity (averaging 7.1 alleles/locus) and heterozygosity (averaging 58.6 %). Allelic diversity at seven loci was sufficient to produce unique multilocus genotypes; no indication of selfing was detected in this cosexual species. Minimal deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium were observed within subpopulations. All loci deviated from Hardy–Weinberg expectations when individuals from subpopulations were pooled. Microsatellite markers developed for T. platysayoides yielded sufficient genetic diversity to (1) distinguish all individuals sampled and the level of selfing; (2) be appropriate for addressing fine-scale population structuring; (3) provide novel demographic insights for the species; and (4) cross-amplify and detect allelic diversity in the congeneric T. juxtidens.

Original language	English (US)
Pages (from-to)	767-769
Number of pages	3
Journal	Conservation Genetics Resources
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/s12686-015-0456-0
State	Published - Sep 18 2015

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Genetics

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title = "Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides",

abstract = "The hermaphroditic flat-spired three-tooth land snail (Triodopsis platysayoides) is endemic to a 21-km stretch of the Cheat River Gorge of northeastern West Virginia, USA. We document isolation and characterization of ten microsatellite DNA markers in this at-risk species. The markers displayed a moderate level of allelic diversity (averaging 7.1 alleles/locus) and heterozygosity (averaging 58.6 %). Allelic diversity at seven loci was sufficient to produce unique multilocus genotypes; no indication of selfing was detected in this cosexual species. Minimal deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium were observed within subpopulations. All loci deviated from Hardy–Weinberg expectations when individuals from subpopulations were pooled. Microsatellite markers developed for T. platysayoides yielded sufficient genetic diversity to (1) distinguish all individuals sampled and the level of selfing; (2) be appropriate for addressing fine-scale population structuring; (3) provide novel demographic insights for the species; and (4) cross-amplify and detect allelic diversity in the congeneric T. juxtidens.",

author = "King, {T. L.} and Eackles, {M. S.} and Garner, {B. A.} and {van Tuinen}, M. and Arbogast, {B. S.}",

note = "Funding Information: This research was funded by the U.S. Geological Survey{\textquoteright}s Leetown Science Center (USGS-LSC), the USGS and U.S. Fish and Wildlife Service{\textquoteright}s Science Support Program, and the West Virginia Division of Natural Resources, Wildlife Resources Section. Use of trade, product, or firm names does not imply endorsement by the U.S. Government. Publisher Copyright: {\textcopyright} 2015, Springer Science+Business Media Dordrecht.",

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doi = "10.1007/s12686-015-0456-0",

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T1 - Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides

AU - King, T. L.

AU - Eackles, M. S.

AU - Garner, B. A.

AU - van Tuinen, M.

AU - Arbogast, B. S.

N1 - Funding Information: This research was funded by the U.S. Geological Survey’s Leetown Science Center (USGS-LSC), the USGS and U.S. Fish and Wildlife Service’s Science Support Program, and the West Virginia Division of Natural Resources, Wildlife Resources Section. Use of trade, product, or firm names does not imply endorsement by the U.S. Government. Publisher Copyright: © 2015, Springer Science+Business Media Dordrecht.

PY - 2015/9/18

Y1 - 2015/9/18

N2 - The hermaphroditic flat-spired three-tooth land snail (Triodopsis platysayoides) is endemic to a 21-km stretch of the Cheat River Gorge of northeastern West Virginia, USA. We document isolation and characterization of ten microsatellite DNA markers in this at-risk species. The markers displayed a moderate level of allelic diversity (averaging 7.1 alleles/locus) and heterozygosity (averaging 58.6 %). Allelic diversity at seven loci was sufficient to produce unique multilocus genotypes; no indication of selfing was detected in this cosexual species. Minimal deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium were observed within subpopulations. All loci deviated from Hardy–Weinberg expectations when individuals from subpopulations were pooled. Microsatellite markers developed for T. platysayoides yielded sufficient genetic diversity to (1) distinguish all individuals sampled and the level of selfing; (2) be appropriate for addressing fine-scale population structuring; (3) provide novel demographic insights for the species; and (4) cross-amplify and detect allelic diversity in the congeneric T. juxtidens.

AB - The hermaphroditic flat-spired three-tooth land snail (Triodopsis platysayoides) is endemic to a 21-km stretch of the Cheat River Gorge of northeastern West Virginia, USA. We document isolation and characterization of ten microsatellite DNA markers in this at-risk species. The markers displayed a moderate level of allelic diversity (averaging 7.1 alleles/locus) and heterozygosity (averaging 58.6 %). Allelic diversity at seven loci was sufficient to produce unique multilocus genotypes; no indication of selfing was detected in this cosexual species. Minimal deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium were observed within subpopulations. All loci deviated from Hardy–Weinberg expectations when individuals from subpopulations were pooled. Microsatellite markers developed for T. platysayoides yielded sufficient genetic diversity to (1) distinguish all individuals sampled and the level of selfing; (2) be appropriate for addressing fine-scale population structuring; (3) provide novel demographic insights for the species; and (4) cross-amplify and detect allelic diversity in the congeneric T. juxtidens.

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Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides

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