Isolation and characterization of microsatellite loci in Alasmidonta heterodon (Bivalvia: Unionidae)

Kristine M. Shaw; Tim L. King; William A. Lellis; Michael S. Eackles

doi:10.1111/j.1471-8286.2005.01235.x

Isolation and characterization of microsatellite loci in Alasmidonta heterodon (Bivalvia: Unionidae)

Kristine M. Shaw, Tim L. King, William A. Lellis, Michael S. Eackles

Ecosystem Science and Management

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7 Scopus citations

Abstract

We developed 13 species-specific microsatellite markers for the federally endangered Atlantic slope unionid Alasmidonta heterodon. Four to 18 alleles per locus were observed among 30 individuals. Observed heterozygosity throughout the loci ranged from 26.9 to 86.2% and averaged 63.6%. Estimates of individual pairwise genetic distances indicated that levels of genetic diversity among loci were sufficient to produce unique multilocus genotypes for all animals surveyed. Randomization tests showed that genotypes for this collection were consistent with Hardy-Weinberg expectations, and no significant linkage disequilibrium was observed between loci. These loci therefore appear suitable for population surveys, kinship assessment and other such applications.

Original language	English (US)
Pages (from-to)	365-367
Number of pages	3
Journal	Molecular Ecology Notes
Volume	6
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2005.01235.x
State	Published - Jun 2006

All Science Journal Classification (ASJC) codes

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

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abstract = "We developed 13 species-specific microsatellite markers for the federally endangered Atlantic slope unionid Alasmidonta heterodon. Four to 18 alleles per locus were observed among 30 individuals. Observed heterozygosity throughout the loci ranged from 26.9 to 86.2% and averaged 63.6%. Estimates of individual pairwise genetic distances indicated that levels of genetic diversity among loci were sufficient to produce unique multilocus genotypes for all animals surveyed. Randomization tests showed that genotypes for this collection were consistent with Hardy-Weinberg expectations, and no significant linkage disequilibrium was observed between loci. These loci therefore appear suitable for population surveys, kinship assessment and other such applications.",

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AB - We developed 13 species-specific microsatellite markers for the federally endangered Atlantic slope unionid Alasmidonta heterodon. Four to 18 alleles per locus were observed among 30 individuals. Observed heterozygosity throughout the loci ranged from 26.9 to 86.2% and averaged 63.6%. Estimates of individual pairwise genetic distances indicated that levels of genetic diversity among loci were sufficient to produce unique multilocus genotypes for all animals surveyed. Randomization tests showed that genotypes for this collection were consistent with Hardy-Weinberg expectations, and no significant linkage disequilibrium was observed between loci. These loci therefore appear suitable for population surveys, kinship assessment and other such applications.

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Isolation and characterization of microsatellite loci in Alasmidonta heterodon (Bivalvia: Unionidae)

Abstract

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