Isolation and characterization of novel tetranucleotide microsatellite DNA markers for the spotted salamander, Ambystoma maculatum

S. E. Julian; Tim L. King; W. K. Savage

doi:10.1046/j.1471-8286.2003.00333.x

Isolation and characterization of novel tetranucleotide microsatellite DNA markers for the spotted salamander, Ambystoma maculatum

S. E. Julian, Tim L. King, W. K. Savage

Ecosystem Science and Management

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Fifteen tetranucleotide microsatellite loci were identified and characterized for spotted salamanders (Ambystoma maculatum) collected from three vernal pools in the southeastern USA. These markers revealed a high degree of genetic diversity (7-32 alleles per locus), heterozygosity (31.6-86.3%) and allelic heterogeneity (91% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.50 and 0.65, and all F_ST values (0.08-0.14) were statistically significant. Moreover, genotypic assignment tests correctly classified all individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and mating system.

Original language	English (US)
Pages (from-to)	7-9
Number of pages	3
Journal	Molecular Ecology Notes
Volume	3
Issue number	1
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00333.x
State	Published - Mar 2003

All Science Journal Classification (ASJC) codes

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

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10.1046/j.1471-8286.2003.00333.x

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abstract = "Fifteen tetranucleotide microsatellite loci were identified and characterized for spotted salamanders (Ambystoma maculatum) collected from three vernal pools in the southeastern USA. These markers revealed a high degree of genetic diversity (7-32 alleles per locus), heterozygosity (31.6-86.3%) and allelic heterogeneity (91% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.50 and 0.65, and all FST values (0.08-0.14) were statistically significant. Moreover, genotypic assignment tests correctly classified all individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and mating system.",

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Isolation and characterization of novel tetranucleotide microsatellite DNA markers for the spotted salamander, Ambystoma maculatum

Abstract

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