Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene

J. Yun Tso; Xiao Hong Sun; Teh Hui Kao; Kimberly S. Reece; Ray Wu

doi:10.1093/nar/13.7.2485

Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene

J. Yun Tso, Xiao Hong Sun, Teh Hui Kao, Kimberly S. Reece, Ray Wu

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

1896 Scopus citations

Abstract

Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome, Some of these realted sequences have been shown to be thesepseudogenes indicating that some pseudogenes are transcrined. Rat and human cDNA are 89% homologous in the coding region, and 76% homologous in the frist 100 base paris of the 3' -noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophils and yeast genes allows analysis of the evolution of the GAPDH genes in detail.

Original language	English (US)
Pages (from-to)	2485-2502
Number of pages	18
Journal	Nucleic acids research
Volume	13
Issue number	7
DOIs	https://doi.org/10.1093/nar/13.7.2485
State	Published - Apr 11 1985

All Science Journal Classification (ASJC) codes

Genetics

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title = "Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene",

abstract = "Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome, Some of these realted sequences have been shown to be thesepseudogenes indicating that some pseudogenes are transcrined. Rat and human cDNA are 89% homologous in the coding region, and 76% homologous in the frist 100 base paris of the 3' -noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophils and yeast genes allows analysis of the evolution of the GAPDH genes in detail.",

author = "Tso, {J. Yun} and Sun, {Xiao Hong} and Kao, {Teh Hui} and Reece, {Kimberly S.} and Ray Wu",

note = "Funding Information: ACKNOWLEDGEMENT We thank Drs. Jack Dixon, Richard Hynes, Savio Woo and Larry Kedes for sending us various cDNA libraries. We also thank Dr. Bruce Paterson for sending us the partial chicken GAPDH cDNA clone. The excellent technical assistance of Bruce Blakely and valuable criticism of the manuscript by Dr. Betty Keller are appreciated. This research was supported by research grant GM29179 from the National Institutes of Health, U.S. Public Health Service.",

year = "1985",

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doi = "10.1093/nar/13.7.2485",

language = "English (US)",

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AU - Tso, J. Yun

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AU - Kao, Teh Hui

AU - Reece, Kimberly S.

AU - Wu, Ray

N1 - Funding Information: ACKNOWLEDGEMENT We thank Drs. Jack Dixon, Richard Hynes, Savio Woo and Larry Kedes for sending us various cDNA libraries. We also thank Dr. Bruce Paterson for sending us the partial chicken GAPDH cDNA clone. The excellent technical assistance of Bruce Blakely and valuable criticism of the manuscript by Dr. Betty Keller are appreciated. This research was supported by research grant GM29179 from the National Institutes of Health, U.S. Public Health Service.

PY - 1985/4/11

Y1 - 1985/4/11

N2 - Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome, Some of these realted sequences have been shown to be thesepseudogenes indicating that some pseudogenes are transcrined. Rat and human cDNA are 89% homologous in the coding region, and 76% homologous in the frist 100 base paris of the 3' -noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophils and yeast genes allows analysis of the evolution of the GAPDH genes in detail.

AB - Full length cDns encoding the glycolyti enzme glyceraldehyde-3-phosphate dehyrogense (GAPDH)fron rat man have been in both genomes based on genmic blot hybridization analysis. Only one functional gene product is know. Result fron genomic library screeings suggest that are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome, Some of these realted sequences have been shown to be thesepseudogenes indicating that some pseudogenes are transcrined. Rat and human cDNA are 89% homologous in the coding region, and 76% homologous in the frist 100 base paris of the 3' -noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophils and yeast genes allows analysis of the evolution of the GAPDH genes in detail.

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Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene

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