Isolation and characterization of the Drosophila melanogaster eIF-2α gene encoding the alpha subunit of translation initiation factor eIF-2

Shimian Qu; Douglas R. Cavener

doi:10.1016/0378-1119(94)90550-9

Isolation and characterization of the Drosophila melanogaster eIF-2α gene encoding the alpha subunit of translation initiation factor eIF-2

Shimian Qu, Douglas R. Cavener

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Abstract

Genomic and cDNA clones encoding the Drosophila melanogaster α-subunit of translational initiation factor 2 (eIF-2α) were isolated. The D. melanogaster eIF-2α gene encodes a 341 amino-acid (aa) protein that shares 57 and 44% identity to its human and yeast homologues, respectively. The regulatory phosphorylation site at Ser⁵⁰ is embedded in a segment of 19 conserved aa residues. Analysis of the genomic DNA and cDNA clones indicated that eIF-2α is a single-copy gene and its coding region is interrupted by a 260-bp intron. The D. melanogaster eIF-2α mRNA is 1350 nt in length and is expressed throughout development.

Original language	English (US)
Pages (from-to)	239-242
Number of pages	4
Journal	Gene
Volume	140
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90550-9
State	Published - Mar 25 1994

All Science Journal Classification (ASJC) codes

Genetics

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10.1016/0378-1119(94)90550-9

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abstract = "Genomic and cDNA clones encoding the Drosophila melanogaster α-subunit of translational initiation factor 2 (eIF-2α) were isolated. The D. melanogaster eIF-2α gene encodes a 341 amino-acid (aa) protein that shares 57 and 44% identity to its human and yeast homologues, respectively. The regulatory phosphorylation site at Ser50 is embedded in a segment of 19 conserved aa residues. Analysis of the genomic DNA and cDNA clones indicated that eIF-2α is a single-copy gene and its coding region is interrupted by a 260-bp intron. The D. melanogaster eIF-2α mRNA is 1350 nt in length and is expressed throughout development.",

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N2 - Genomic and cDNA clones encoding the Drosophila melanogaster α-subunit of translational initiation factor 2 (eIF-2α) were isolated. The D. melanogaster eIF-2α gene encodes a 341 amino-acid (aa) protein that shares 57 and 44% identity to its human and yeast homologues, respectively. The regulatory phosphorylation site at Ser50 is embedded in a segment of 19 conserved aa residues. Analysis of the genomic DNA and cDNA clones indicated that eIF-2α is a single-copy gene and its coding region is interrupted by a 260-bp intron. The D. melanogaster eIF-2α mRNA is 1350 nt in length and is expressed throughout development.

AB - Genomic and cDNA clones encoding the Drosophila melanogaster α-subunit of translational initiation factor 2 (eIF-2α) were isolated. The D. melanogaster eIF-2α gene encodes a 341 amino-acid (aa) protein that shares 57 and 44% identity to its human and yeast homologues, respectively. The regulatory phosphorylation site at Ser50 is embedded in a segment of 19 conserved aa residues. Analysis of the genomic DNA and cDNA clones indicated that eIF-2α is a single-copy gene and its coding region is interrupted by a 260-bp intron. The D. melanogaster eIF-2α mRNA is 1350 nt in length and is expressed throughout development.

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Isolation and characterization of the Drosophila melanogaster eIF-2α gene encoding the alpha subunit of translation initiation factor eIF-2

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