Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci

Sarah K. Bronson; J. I. Pei; Patricia Taillon-Miller; Michael J. Chorney; Daniel E. Geraghty; David D. Chaplin

doi:10.1073/pnas.88.5.1676

Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci

Sarah K. Bronson, J. I. Pei, Patricia Taillon-Miller, Michael J. Chorney, Daniel E. Geraghty, David D. Chaplin

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Abstract

A 290-kilobase-pair chromosomal segment containing the genes encoding the human class 1 major histocompatibility complex molecules HLA-B and HLA-C as well as a class 1 pseudogene has been isolated on three overlapping yeast artificial chromosome (YAC) clones. One YAC clone contains both the HLA-B and HLA-C genes. These loci are located ≈85 kilobase pairs apart, each in close association with a CpG island. Southern blotting and nucleotide sequencing showed no evidence of alteration of the structure of the cloned DNA in the YACs. End fragments from the YAC inserts have been isolated and used to confirm the overlaps between clones. These fragments can also serve as polymorphic markers for structural analysis of the major histocompatibility complex. Our data show that YAC cloning offers an attractive alternative for analysis of the structures of large gene complexes such as HLA.

Original language	English (US)
Pages (from-to)	1676-1680
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	5
DOIs	https://doi.org/10.1073/pnas.88.5.1676
State	Published - 1991

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title = "Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci",

abstract = "A 290-kilobase-pair chromosomal segment containing the genes encoding the human class 1 major histocompatibility complex molecules HLA-B and HLA-C as well as a class 1 pseudogene has been isolated on three overlapping yeast artificial chromosome (YAC) clones. One YAC clone contains both the HLA-B and HLA-C genes. These loci are located ≈85 kilobase pairs apart, each in close association with a CpG island. Southern blotting and nucleotide sequencing showed no evidence of alteration of the structure of the cloned DNA in the YACs. End fragments from the YAC inserts have been isolated and used to confirm the overlaps between clones. These fragments can also serve as polymorphic markers for structural analysis of the major histocompatibility complex. Our data show that YAC cloning offers an attractive alternative for analysis of the structures of large gene complexes such as HLA.",

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T1 - Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci

AU - Bronson, Sarah K.

AU - Pei, J. I.

AU - Taillon-Miller, Patricia

AU - Chorney, Michael J.

AU - Geraghty, Daniel E.

AU - Chaplin, David D.

PY - 1991

Y1 - 1991

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AB - A 290-kilobase-pair chromosomal segment containing the genes encoding the human class 1 major histocompatibility complex molecules HLA-B and HLA-C as well as a class 1 pseudogene has been isolated on three overlapping yeast artificial chromosome (YAC) clones. One YAC clone contains both the HLA-B and HLA-C genes. These loci are located ≈85 kilobase pairs apart, each in close association with a CpG island. Southern blotting and nucleotide sequencing showed no evidence of alteration of the structure of the cloned DNA in the YACs. End fragments from the YAC inserts have been isolated and used to confirm the overlaps between clones. These fragments can also serve as polymorphic markers for structural analysis of the major histocompatibility complex. Our data show that YAC cloning offers an attractive alternative for analysis of the structures of large gene complexes such as HLA.

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Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci

Abstract

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