TY - JOUR
T1 - Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family
AU - Aapola, Ulla
AU - Shibuya, Kazunori
AU - Scott, Hamish S.
AU - Ollila, Juha
AU - Vihinen, Mauno
AU - Heino, Maarit
AU - Shintani, Ai
AU - Kawasaki, Kazuhiko
AU - Minoshima, Shinsei
AU - Krohn, Kai
AU - Antonarakis, Stylianos E.
AU - Shimizu, Nobuyoshi
AU - Kudoh, Jun
AU - Peterson, Pärt
N1 - Funding Information:
The help of Ms. Ulla Kiiskinen is highly appreciated. We thank the members of the sequencing team at the Keio University School of Medicine. U.A., M.H., M.V., K.K., and P.P. were supported by grants from Tampere University Hospital Medical Fund (99186, 98191) and the Finnish Academy (45247, 46066); H.S.S. and S.E.A. were supported by Swiss FNRS 31-40500.94 and the European Union/OFES 97-0015 and funds from the University and Cantonal Hospital of Geneva. K.S., J.K., A.S., K.K., S.M., and N.S. were supported by Funds for the Human Genome Sequencing Project from the Japan Science and Technology Corp. (JST), Grants in Aid for Scientific Research on Priority Areas and Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan, and the Fund for “Research for the Future” Program from the Japan Society for the Promotion of Science (JSPS).
PY - 2000/5/1
Y1 - 2000/5/1
N2 - We have isolated the DNMT3L gene that is related to the cytosine-5- methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family. The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.
AB - We have isolated the DNMT3L gene that is related to the cytosine-5- methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family. The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.
UR - https://www.scopus.com/pages/publications/18744427037
UR - https://www.scopus.com/pages/publications/18744427037#tab=citedBy
U2 - 10.1006/geno.2000.6168
DO - 10.1006/geno.2000.6168
M3 - Article
C2 - 10857753
AN - SCOPUS:18744427037
SN - 0888-7543
VL - 65
SP - 293
EP - 298
JO - Genomics
JF - Genomics
IS - 3
ER -