TY - JOUR
T1 - Isolation and maintenance of Batrachochytrium salamandrivorans cultures
AU - Robinson, Kristyn A.
AU - Pereira, Kenzie E.
AU - Bletz, Molly C.
AU - Carter, Edward Davis
AU - Gray, Matthew J.
AU - Piovia-Scott, Jonah
AU - Romansic, John M.
AU - Woodhams, Douglas C.
AU - Fritz-Laylin, Lillian
N1 - Publisher Copyright:
© The authors 2020. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are unrestricted. Authors and original publication must be credited.
PY - 2020
Y1 - 2020
N2 - Discovered in 2013, the chytrid fungus Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, B. dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a medium for standardized Bsal growth, standard culturing protocols, and a method for isolating Bsal from infected tissue.
AB - Discovered in 2013, the chytrid fungus Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, B. dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a medium for standardized Bsal growth, standard culturing protocols, and a method for isolating Bsal from infected tissue.
UR - https://www.scopus.com/pages/publications/85087472287
UR - https://www.scopus.com/inward/citedby.url?scp=85087472287&partnerID=8YFLogxK
U2 - 10.3354/DAO03488
DO - 10.3354/DAO03488
M3 - Article
C2 - 32618283
AN - SCOPUS:85087472287
SN - 0177-5103
VL - 140
SP - 1
EP - 11
JO - Diseases of Aquatic Organisms
JF - Diseases of Aquatic Organisms
ER -