TY - JOUR
T1 - Isolation and molecular characterization of newly emerging avian reovirus variants and novel strains in Pennsylvania, USA, 2011-2014
AU - Lu, Huaguang
AU - Tang, Yi
AU - Dunn, Patricia A.
AU - Wallner-Pendleton, Eva A.
AU - Lin, Lin
AU - Knoll, Eric A.
N1 - Funding Information:
These avian reovirus diagnostic and research studies were funded by The Pennsylvania Department of Agriculture (PDA), Animal Health and Diagnostic Commission (AHDC)’s Research Program, 2013– 2015, Pennsylvania, USA; The Pennsylvania Poultry Industry Broiler/Egg Check-Off Research Program and The Pennsylvania Soybean Board Research Program, 2015, Pennsylvania, USA.
PY - 2015/10/15
Y1 - 2015/10/15
N2 - Avian reovirus (ARV) infections of broiler and Turkey flocks have caused significant clinical disease and economic losses in Pennsylvania (PA) since 2011. Most of the ARV-infected birds suffered from severe arthritis, tenosynovitis, pericarditis and depressed growth or runting-stunting syndrome (RSS). A high morbidity (up to 20% to 40%) was observed in ARV-affected flocks, and the flock mortality was occasionally as high as 10%. ARV infections in Turkeys were diagnosed for the first time in PA in 2011. From 2011 to 2014, a total of 301 ARV isolations were made from affected PA poultry. The molecular characterization of the Sigma C gene of 114 field isolates, representing most ARV outbreaks, revealed that only 21.93% of the 114 sequenced ARV isolates were in the same genotyping cluster (cluster 1) as the ARV vaccine strains (S1133, 1733, and 2048), whereas 78.07% of the sequenced isolates were in genotyping clusters 2, 3, 4, 5, and 6 (which were distinct from the vaccine strains) and represented newly emerging ARV variants. In particular, genotyping cluster 6 was a new ARV genotype that was identified for the first time in 10 novel PA ARV variants of field isolates.
AB - Avian reovirus (ARV) infections of broiler and Turkey flocks have caused significant clinical disease and economic losses in Pennsylvania (PA) since 2011. Most of the ARV-infected birds suffered from severe arthritis, tenosynovitis, pericarditis and depressed growth or runting-stunting syndrome (RSS). A high morbidity (up to 20% to 40%) was observed in ARV-affected flocks, and the flock mortality was occasionally as high as 10%. ARV infections in Turkeys were diagnosed for the first time in PA in 2011. From 2011 to 2014, a total of 301 ARV isolations were made from affected PA poultry. The molecular characterization of the Sigma C gene of 114 field isolates, representing most ARV outbreaks, revealed that only 21.93% of the 114 sequenced ARV isolates were in the same genotyping cluster (cluster 1) as the ARV vaccine strains (S1133, 1733, and 2048), whereas 78.07% of the sequenced isolates were in genotyping clusters 2, 3, 4, 5, and 6 (which were distinct from the vaccine strains) and represented newly emerging ARV variants. In particular, genotyping cluster 6 was a new ARV genotype that was identified for the first time in 10 novel PA ARV variants of field isolates.
UR - https://www.scopus.com/pages/publications/84944323260
UR - https://www.scopus.com/pages/publications/84944323260#tab=citedBy
U2 - 10.1038/srep14727
DO - 10.1038/srep14727
M3 - Article
C2 - 26469681
AN - SCOPUS:84944323260
SN - 2045-2322
VL - 5
JO - Scientific reports
JF - Scientific reports
M1 - 14727
ER -