TY - JOUR
T1 - Isolation and profiling of plasma microRNAs
T2 - Biomarkers for asthma and allergic rhinitis
AU - Panganiban, Ronaldo P.
AU - Lambert, Kristin A.
AU - Hsu, Man Hsun
AU - Laryea, Zoe
AU - Ishmael, Faoud T.
N1 - Funding Information:
This work was supported by the Doris Duke Charitable Foundation Clinician Scientist Development Award.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility.
AB - Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility.
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U2 - 10.1016/j.ymeth.2018.06.007
DO - 10.1016/j.ymeth.2018.06.007
M3 - Article
C2 - 29906503
AN - SCOPUS:85049758168
SN - 1046-2023
VL - 152
SP - 48
EP - 54
JO - Methods
JF - Methods
ER -
