Isolation and profiling of plasma microRNAs: Biomarkers for asthma and allergic rhinitis

Ronaldo P. Panganiban; Kristin A. Lambert; Man Hsun Hsu; Zoe Laryea; Faoud Ishmael

doi:10.1016/j.ymeth.2018.06.007

Isolation and profiling of plasma microRNAs: Biomarkers for asthma and allergic rhinitis

Ronaldo P. Panganiban
, Kristin A. Lambert
, Man Hsun Hsu
, Zoe Laryea
, Faoud Ishmael

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility.

Original language	English (US)
Pages (from-to)	48-54
Number of pages	7
Journal	Methods
Volume	152
DOIs	https://doi.org/10.1016/j.ymeth.2018.06.007
State	Published - Jan 1 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.ymeth.2018.06.007

Cite this

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title = "Isolation and profiling of plasma microRNAs: Biomarkers for asthma and allergic rhinitis",

abstract = "Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility.",

author = "Panganiban, \{Ronaldo P.\} and Lambert, \{Kristin A.\} and Hsu, \{Man Hsun\} and Zoe Laryea and Faoud Ishmael",

note = "Funding Information: This work was supported by the Doris Duke Charitable Foundation Clinician Scientist Development Award. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

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doi = "10.1016/j.ymeth.2018.06.007",

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T2 - Biomarkers for asthma and allergic rhinitis

AU - Panganiban, Ronaldo P.

AU - Lambert, Kristin A.

AU - Hsu, Man Hsun

AU - Laryea, Zoe

AU - Ishmael, Faoud

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility.

AB - Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility.

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JO - Methods

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ER -

Isolation and profiling of plasma microRNAs: Biomarkers for asthma and allergic rhinitis

Abstract

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