TY - JOUR
T1 - Isolation and sequence analysis of TGA1 cDNAs encoding a tomato G protein α subunit
AU - Hong, Ma
AU - Yanofsky, Martin F.
AU - Hai, Huang
N1 - Funding Information:
We thank A. Tagle for technical assistance,C .S. Gassar for generously supplying the tomato cDNA library, E. Richards for providing tomato genomic DNA, R. Martienssen and E. Richards for helpful discussion and comments on this manuscript. This work was supportedb y a grant from Pioneer Hi-bred International and funds from the Robertson Foundation to H.M., and by an award from the David and Lucile Packard Foundation and funds from the Powell Foundation to M.F.Y.
PY - 1991/11/15
Y1 - 1991/11/15
N2 - We have isolated cDNAs for a gene coding for a G protein a subunit from tomato (Lycopersicon esculentum, cv. VF36). This gene, named TGA1, was isolated using a cDNA of the Arabidopsis thaliana G protein a subunit-encoding gene, GPA1, as a DNA probe. The sequences of four cDNA clones indicate that the deduced amino acid (aa) sequence of the gene product (TGal) has 384 aa (44906 Da). The predicted TGαl protein exhibits similarity to all known G protein α subunits. The aa are 84.6% identical and 93% similar (identical and conservative changes) to A. thaliana GPαl, and 34% identical and 59% similar to mammalian transducins. Furthermore, it has all of the consensus regions for a GTP-binding protein. Finally, hybridizations of tomato genomic DNA indicate that TGA1 is a single-copy gene.
AB - We have isolated cDNAs for a gene coding for a G protein a subunit from tomato (Lycopersicon esculentum, cv. VF36). This gene, named TGA1, was isolated using a cDNA of the Arabidopsis thaliana G protein a subunit-encoding gene, GPA1, as a DNA probe. The sequences of four cDNA clones indicate that the deduced amino acid (aa) sequence of the gene product (TGal) has 384 aa (44906 Da). The predicted TGαl protein exhibits similarity to all known G protein α subunits. The aa are 84.6% identical and 93% similar (identical and conservative changes) to A. thaliana GPαl, and 34% identical and 59% similar to mammalian transducins. Furthermore, it has all of the consensus regions for a GTP-binding protein. Finally, hybridizations of tomato genomic DNA indicate that TGA1 is a single-copy gene.
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U2 - 10.1016/0378-1119(91)90318-6
DO - 10.1016/0378-1119(91)90318-6
M3 - Article
C2 - 1748292
AN - SCOPUS:0026091449
SN - 0378-1119
VL - 107
SP - 189
EP - 195
JO - Gene
JF - Gene
IS - 2
ER -