TY - JOUR
T1 - Isolation, cloning, and characterization of a novel rat lung zinc finger gene, RLZF-Y
AU - Dovat, Sinisa
AU - Gilbert, Kirk A.
AU - Petrovic-Dovat, Lidija
AU - Rannels, D. Eugene
N1 - Funding Information:
The authors thank Suzanne P. Sass-Kuhn and Cara Martinez-Williams for excellent technical assistance. This work was supported by HL-20344 (D.E.R.) and HL-08954 (K.A.G.) from the National Heart, Lung and Blood Institute of the National Institutes of Health. The nucleotide sequence for RLZF-Y can be found in the GenBank database (GenBank accession no. AF052042).
PY - 1998/11/8
Y1 - 1998/11/8
N2 - Zinc-finger (ZF) proteins are widely distributed. The current study reports isolation, cloning and characterization of a novel ZF gene, RLZF-Y. Total RNA from rat lung was reverse transcribed. The 5' and 3' ends were isolated by rapid amplification of cDNA ends (RACE) using primers derived from a previously isolated partial clone. RACE products of 1.5 and 1.1 kb were cloned and sequenced. Identical overlapping sequence of 70 base pairs confirmed representation of the same cDNA approximately 2.5 kb in length. Probes derived from both 5' RACE and 3' RACE products independently hybridized to a 2.5 kb mRNA from rat lung. RLZF-Y mRNA is expressed in lung, brain, heart and kidney; expression is low in liver. Predicted amino acid sequence analysis defined three regions of similarity to known C2H2 ZF proteins: a region containing seven ZF structures characteristic of the Kruppel-like subfamily of ZF genes; a region with sequence similarity to the Kruppel-associated box A (KRAB-A) domain at the amino end; an amino-terminal leucine-rich region (LeR) adjacent to KRAB-A. The presence of KRAB-A and the adjacent LeR implies RLZF-Y protein may function as a transcriptional repressor.
AB - Zinc-finger (ZF) proteins are widely distributed. The current study reports isolation, cloning and characterization of a novel ZF gene, RLZF-Y. Total RNA from rat lung was reverse transcribed. The 5' and 3' ends were isolated by rapid amplification of cDNA ends (RACE) using primers derived from a previously isolated partial clone. RACE products of 1.5 and 1.1 kb were cloned and sequenced. Identical overlapping sequence of 70 base pairs confirmed representation of the same cDNA approximately 2.5 kb in length. Probes derived from both 5' RACE and 3' RACE products independently hybridized to a 2.5 kb mRNA from rat lung. RLZF-Y mRNA is expressed in lung, brain, heart and kidney; expression is low in liver. Predicted amino acid sequence analysis defined three regions of similarity to known C2H2 ZF proteins: a region containing seven ZF structures characteristic of the Kruppel-like subfamily of ZF genes; a region with sequence similarity to the Kruppel-associated box A (KRAB-A) domain at the amino end; an amino-terminal leucine-rich region (LeR) adjacent to KRAB-A. The presence of KRAB-A and the adjacent LeR implies RLZF-Y protein may function as a transcriptional repressor.
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U2 - 10.1016/S0167-4781(98)00167-5
DO - 10.1016/S0167-4781(98)00167-5
M3 - Article
C2 - 9804994
AN - SCOPUS:0032497803
SN - 0167-4781
VL - 1442
SP - 380
EP - 388
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 2-3
ER -