Isolation of both normal and leukemic cells within the hematopoietic stem cell compartment of chronic myelogenous leukemia mobilized peripheral blood

A. S. Tsukamoto, D. Van Den Berg, C. Reading, J. Tong, L. Murray, A. Carella, F. Frassoni, D. Snyder, G. Herzig, C. Gorin, J. Laporte, R. Negrin, R. Blume, I. Cunningham, D. Claxton, A. Deisseroth, R. Hoffman

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chronic myelogenous leukemia has long been thought to be hematologic malignancy of stem cells characterized by the presence of the Philadelphia chromosome. Significant advances have been made in understanding molecular and biological abnormalities resulting from this chromosomal translocation. The detection and isolation of benign stem cells, devoid of the bcr-abl translocation from MPB samples of CML patients supports both clinical and experimental observations of the presence of normal hematopoietic cells in in vivo and in vitro cultures. Detection of the hybridized probes was performed using FITC (green) and Rhodamine (red) labeled secondary reagents (avidin or an anti-digoxigenin monoclonal antibody.) Nuclear DNA was counterstained and microscopy was performed to determine the number and location of the fluorescent signals. The detection and isolation of benign stem cells, devoid of the bcr-abl translocation from mobilized peripheral blood samples of Chronic myelogenous leukemia patients supports both clinical and experimental observations of the presence of normal hematopoietic cells in in vivo and in vitro cultures.

Original languageEnglish (US)
Title of host publicationAutologous Stem Cell Transplantation
Subtitle of host publicationBiological and Clinical Results in Malignancies
PublisherTaylor and Francis
Pages27-32
Number of pages6
ISBN (Electronic)9781000102826
DOIs
StatePublished - Jan 1 2020

All Science Journal Classification (ASJC) codes

  • General Social Sciences

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