TY - JOUR
T1 - Isolation of candidate hybrid sterility 1 genes by cDNA selection in a 1.1 megabase pair region on mouse Chromosome 17
AU - Trachtulec, Zdenek
AU - Mňuková-Fajdelová, Monika
AU - Hamvas, Renata M.J.
AU - Gregorová, Soña
AU - Mayer, Werner E.
AU - Lehrach, Hans R.
AU - Vincek, Vladimir
AU - Forejt, Jiří
AU - Klein, Jan
PY - 1997
Y1 - 1997
N2 - The Hybrid sterility 1 (Hst1) gene causes male infertility in crosses between certain inbred strains of the laboratory and wild mouse, Mus musculus. To identify the causative gene, we have searched YAC clones encompassing the Hst1 region for testis-expressed sequences, using the cDNA selection method. We isolated 12 non-overlapping cDNA clones, sequenced them, and placed them on a physical map based on the analysis of YAC clones and total genomic DNA. The cDNA clones map to ten loci. Three cDNA sequences correspond to the proteasome subunit C5 (locus Psmb1), ornithine decarboxylase (Odc-rs15), and pentazinc finger (Zfp91-rs1) transcripts. Three of the ten testis-expressed loci described in this report (D17Ph4e, Psmb1, and Zfp91-rsl) co-segregate with all Hst1 recombinants and, together with the Tbp gene, are therefore potential candidates for the Hst1 gene. The presented physical and genetic mapping data indicate there are no gross rearrangements distinguishing the Hstl(f) and Hstl(s) alleles.
AB - The Hybrid sterility 1 (Hst1) gene causes male infertility in crosses between certain inbred strains of the laboratory and wild mouse, Mus musculus. To identify the causative gene, we have searched YAC clones encompassing the Hst1 region for testis-expressed sequences, using the cDNA selection method. We isolated 12 non-overlapping cDNA clones, sequenced them, and placed them on a physical map based on the analysis of YAC clones and total genomic DNA. The cDNA clones map to ten loci. Three cDNA sequences correspond to the proteasome subunit C5 (locus Psmb1), ornithine decarboxylase (Odc-rs15), and pentazinc finger (Zfp91-rs1) transcripts. Three of the ten testis-expressed loci described in this report (D17Ph4e, Psmb1, and Zfp91-rsl) co-segregate with all Hst1 recombinants and, together with the Tbp gene, are therefore potential candidates for the Hst1 gene. The presented physical and genetic mapping data indicate there are no gross rearrangements distinguishing the Hstl(f) and Hstl(s) alleles.
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U2 - 10.1007/s003359900430
DO - 10.1007/s003359900430
M3 - Article
C2 - 9107673
AN - SCOPUS:0031136320
SN - 0938-8990
VL - 8
SP - 312
EP - 316
JO - Mammalian Genome
JF - Mammalian Genome
IS - 5
ER -