Isolation of the intact photosystem I reaction center core containing P700 and iron-sulfur center FX

John H. Golbeck; Kevin G. Parrett; Tetemke Mehari; Karen L. Jones; Jerry J. Brand

doi:10.1016/0014-5793(88)80013-9

Isolation of the intact photosystem I reaction center core containing P700 and iron-sulfur center F_X

John H. Golbeck, Kevin G. Parrett, Tetemke Mehari, Karen L. Jones, Jerry J. Brand

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

The photosystem I reaction center core containing P700 and iron-sulfur center F_X has been isolated from a Synechococcus photosystem I particle with 6.8 M urea at pH 10.0 followed by sucrose density ultracentrifugation. The reaction center core has retained > 90% of F_X and 100% of P700 (determined by optical spectroscopy) but is totally devoid of iron-sulfur centers F_A and F_B (determined by optical and ESR spectroscopy). SDS-PAGE indicates the retention of the 57 kDa reaction center polypeptide(s) but the total absence of the 16.4 and 8.1 kDa polypeptides. The loss of F_A and F_B is further reflected in the decline of acid-labile sulfide from 11.8 ± 0.4 S^2- /P700 in the control particle to 4.6 ± 0.3 S^2- /P700 in the reaction center core. This preparation represents the first isolation of an intact reaction center core incorporating the components P700 and F_X but totally lacking F_A and F_B.

Original language	English (US)
Pages (from-to)	268-272
Number of pages	5
Journal	FEBS Letters
Volume	228
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(88)80013-9
State	Published - Feb 15 1988

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(88)80013-9

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title = "Isolation of the intact photosystem I reaction center core containing P700 and iron-sulfur center FX",

abstract = "The photosystem I reaction center core containing P700 and iron-sulfur center FX has been isolated from a Synechococcus photosystem I particle with 6.8 M urea at pH 10.0 followed by sucrose density ultracentrifugation. The reaction center core has retained > 90% of FX and 100% of P700 (determined by optical spectroscopy) but is totally devoid of iron-sulfur centers FA and FB (determined by optical and ESR spectroscopy). SDS-PAGE indicates the retention of the 57 kDa reaction center polypeptide(s) but the total absence of the 16.4 and 8.1 kDa polypeptides. The loss of FA and FB is further reflected in the decline of acid-labile sulfide from 11.8 ± 0.4 S2- /P700 in the control particle to 4.6 ± 0.3 S2- /P700 in the reaction center core. This preparation represents the first isolation of an intact reaction center core incorporating the components P700 and FX but totally lacking FA and FB.",

author = "Golbeck, {John H.} and Parrett, {Kevin G.} and Tetemke Mehari and Jones, {Karen L.} and Brand, {Jerry J.}",

note = "Funding Information: AcknowledgementsT:h is materiali s based upon work sup-portedb y the CooperativeS tateR esearchS ervice,U S Depart-mento f Agricultureu nderA greemennt o. 87-CRCR-1-238(2t o J.H.G.), and by a grantf rom the RobertA . WelchF oundation, no. F-867 (to J.J.B.).",

year = "1988",

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doi = "10.1016/0014-5793(88)80013-9",

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T1 - Isolation of the intact photosystem I reaction center core containing P700 and iron-sulfur center FX

AU - Golbeck, John H.

AU - Parrett, Kevin G.

AU - Mehari, Tetemke

AU - Jones, Karen L.

AU - Brand, Jerry J.

N1 - Funding Information: AcknowledgementsT:h is materiali s based upon work sup-portedb y the CooperativeS tateR esearchS ervice,U S Depart-mento f Agricultureu nderA greemennt o. 87-CRCR-1-238(2t o J.H.G.), and by a grantf rom the RobertA . WelchF oundation, no. F-867 (to J.J.B.).

PY - 1988/2/15

Y1 - 1988/2/15

N2 - The photosystem I reaction center core containing P700 and iron-sulfur center FX has been isolated from a Synechococcus photosystem I particle with 6.8 M urea at pH 10.0 followed by sucrose density ultracentrifugation. The reaction center core has retained > 90% of FX and 100% of P700 (determined by optical spectroscopy) but is totally devoid of iron-sulfur centers FA and FB (determined by optical and ESR spectroscopy). SDS-PAGE indicates the retention of the 57 kDa reaction center polypeptide(s) but the total absence of the 16.4 and 8.1 kDa polypeptides. The loss of FA and FB is further reflected in the decline of acid-labile sulfide from 11.8 ± 0.4 S2- /P700 in the control particle to 4.6 ± 0.3 S2- /P700 in the reaction center core. This preparation represents the first isolation of an intact reaction center core incorporating the components P700 and FX but totally lacking FA and FB.

AB - The photosystem I reaction center core containing P700 and iron-sulfur center FX has been isolated from a Synechococcus photosystem I particle with 6.8 M urea at pH 10.0 followed by sucrose density ultracentrifugation. The reaction center core has retained > 90% of FX and 100% of P700 (determined by optical spectroscopy) but is totally devoid of iron-sulfur centers FA and FB (determined by optical and ESR spectroscopy). SDS-PAGE indicates the retention of the 57 kDa reaction center polypeptide(s) but the total absence of the 16.4 and 8.1 kDa polypeptides. The loss of FA and FB is further reflected in the decline of acid-labile sulfide from 11.8 ± 0.4 S2- /P700 in the control particle to 4.6 ± 0.3 S2- /P700 in the reaction center core. This preparation represents the first isolation of an intact reaction center core incorporating the components P700 and FX but totally lacking FA and FB.

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Isolation of the intact photosystem I reaction center core containing P700 and iron-sulfur center F_X

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