JIL-1: A novel chromosomal tandem kinase implicated in transcriptional regulation in Drosophila

Ye Jin, Yanming Wang, Diana L. Walker, Hao Dong, Cherice Conley, Jørgen Johansen, Kristen M. Johansen

Research output: Contribution to journalArticlepeer-review

141 Scopus citations

Abstract

We have cloned and characterized JIL-1, a novel tandem kinase in Drosophila that associates with the chromosomes throughout the cell cycle. Antibody staining and live imaging of JIL-1-GFP transgenic flies show that JIL-1 localizes to the gene-rich interband regions of larval polytene chromosomes and is upregulated almost 2-fold on the hypertranscribed male X chromosome compared to autosomes. Phylogenetic analysis suggests that JIL-1 together with human MSKs defines a separate family of tandem kinases. That JIL-1 is a functional kinase was demonstrated by autophosphorylation and phosphorylation of histone H3 in vitro. Based on these findings, we propose that JIL-1 may play a role in transcriptional control potentially by regulating chromatin structure.

Original languageEnglish (US)
Pages (from-to)129-135
Number of pages7
JournalMolecular cell
Volume4
Issue number1
DOIs
StatePublished - Jul 1999

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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