Kinetic and Dynamic Effects on Degradation of von Willebrand Factor

Choon Sik Jhun, Lichong Xu, Christopher Siedlecki, Carlo R. Bartoli, Eric Yeager, Branka Lukic, Christopher M. Scheib, Raymond Newswanger, Joshua P. Cysyk, Chan Shen, Karl Bohnenberger, William J. Weiss, Gerson Rosenberg

Research output: Contribution to journalArticlepeer-review

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Abstract

The loss of high molecular weight multimers (HMWM) of von Willebrand factor (vWF) in aortic stenosis (AS) and continuous-flow left ventricular assist devices (cf-LVADs) is believed to be associated with high turbulent blood shear. The objective of this study is to understand the degradation mechanism of HMWM in terms of exposure time (kinetic) and flow regime (dynamics) within clinically relevant pathophysiologic conditions. A custom high-shear rotary device capable of creating fully controlled exposure times and flows was used. The system was set so that human platelet-poor plasma flowed through at 1.75 ml/sec, 0.76 ml/sec, or 0.38 ml/sec resulting in the exposure time (texp) of 22, 50, or 100 ms, respectively. The flow was characterized by the Reynolds number (Re). The device was run under laminar (Re = 1,500), transitional (Re = 3,000; Re = 3,500), and turbulent (Re = 4,500) conditions at a given texpfollowed by multimer analysis. No degradation was observed at laminar flow at all given texp. Degradation of HMWM at a given texpincreases with the Re. Re (p < 0.0001) and texp(p = 0.0034) are significant factors in the degradation of HMWM. Interaction between Re and texp, however, is not always significant (p = 0.73).

Original languageEnglish (US)
Pages (from-to)467-474
Number of pages8
JournalASAIO Journal
Volume69
Issue number5
DOIs
StatePublished - May 1 2023

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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