Kinetic and structural comparison of a protein’s cotranslational folding and refolding pathways

Avi J. Samelson, Eric Bolin, Shawn M. Costello, Ajeet K. Sharma, Edward P. O’Brien, Susan Marqusee

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Precise protein folding is essential for the survival of all cells, and protein misfolding causes a number of diseases that lack effective therapies, yet the general principles governing protein folding in the cell remain poorly understood. In vivo, folding can begin cotranslationally and protein quality control at the ribosome is essential for cellular proteostasis. We directly characterize and compare the refolding and cotranslational folding trajectories of the protein HaloTag. We introduce new techniques for both measuring folding kinetics and detecting the conformations of partially folded intermediates during translation in real time. We find that, although translation does not affect the rate-limiting step of HaloTag folding, a key aggregation-prone intermediate observed during in vitro refolding experiments is no longer detectable. This rerouting of the folding pathway increases HaloTag’s folding efficiency and may serve as a general chaperone-independent mechanism of quality control by the ribosome.

Original languageEnglish (US)
Article numbereaas9098
JournalScience Advances
Issue number5
StatePublished - May 30 2018

All Science Journal Classification (ASJC) codes

  • General


Dive into the research topics of 'Kinetic and structural comparison of a protein’s cotranslational folding and refolding pathways'. Together they form a unique fingerprint.

Cite this