Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

Simon A. Cobbold; Ashley M. Vaughan; Ian A. Lewis; Heather J. Painter; Nelly Camargo; David H. Perlman; Matthew Fishbaugher; Julie Healer; Alan F. Cowman; Stefan H.I. Kappe; Manuel Llinás

doi:10.1074/jbc.M113.503557

Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

Simon A. Cobbold, Ashley M. Vaughan, Ian A. Lewis, Heather J. Painter, Nelly Camargo, David H. Perlman, Matthew Fishbaugher, Julie Healer, Alan F. Cowman, Stefan H.I. Kappe, Manuel Llinás

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73 Scopus citations

Abstract

Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.

Original language	English (US)
Pages (from-to)	36338-36350
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	288
Issue number	51
DOIs	https://doi.org/10.1074/jbc.M113.503557
State	Published - Dec 20 2013

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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Cobbold, S. A., Vaughan, A. M., Lewis, I. A., Painter, H. J., Camargo, N., Perlman, D. H., Fishbaugher, M., Healer, J., Cowman, A. F., Kappe, S. H. I., & Llinás, M. (2013). Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum. Journal of Biological Chemistry, 288(51), 36338-36350. https://doi.org/10.1074/jbc.M113.503557

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abstract = "Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.",

author = "Cobbold, {Simon A.} and Vaughan, {Ashley M.} and Lewis, {Ian A.} and Painter, {Heather J.} and Nelly Camargo and Perlman, {David H.} and Matthew Fishbaugher and Julie Healer and Cowman, {Alan F.} and Kappe, {Stefan H.I.} and Manuel Llin{\'a}s",

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doi = "10.1074/jbc.M113.503557",

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T1 - Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

AU - Cobbold, Simon A.

AU - Vaughan, Ashley M.

AU - Lewis, Ian A.

AU - Painter, Heather J.

AU - Camargo, Nelly

AU - Perlman, David H.

AU - Fishbaugher, Matthew

AU - Healer, Julie

AU - Cowman, Alan F.

AU - Kappe, Stefan H.I.

AU - Llinás, Manuel

PY - 2013/12/20

Y1 - 2013/12/20

N2 - Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.

AB - Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.

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Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

Abstract

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