Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

Simon A. Cobbold; Ashley M. Vaughan; Ian A. Lewis; Heather J. Painter; Nelly Camargo; David H. Perlman; Matthew Fishbaugher; Julie Healer; Alan F. Cowman; Stefan H.I. Kappe; Manuel Llinás

doi:10.1074/jbc.M113.503557

Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

Simon A. Cobbold
, Ashley M. Vaughan
, Ian A. Lewis
, Heather J. Painter
, Nelly Camargo
, David H. Perlman
, Matthew Fishbaugher
, Julie Healer
, Alan F. Cowman
, Stefan H.I. Kappe
, Manuel Llinás

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.

Original language	English (US)
Pages (from-to)	36338-36350
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	288
Issue number	51
DOIs	https://doi.org/10.1074/jbc.M113.503557
State	Published - Dec 20 2013

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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Cobbold, S. A., Vaughan, A. M., Lewis, I. A., Painter, H. J., Camargo, N., Perlman, D. H., Fishbaugher, M., Healer, J., Cowman, A. F., Kappe, S. H. I., & Llinás, M. (2013). Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum. Journal of Biological Chemistry, 288(51), 36338-36350. https://doi.org/10.1074/jbc.M113.503557

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title = "Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum",

abstract = "Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.",

author = "Cobbold, \{Simon A.\} and Vaughan, \{Ashley M.\} and Lewis, \{Ian A.\} and Painter, \{Heather J.\} and Nelly Camargo and Perlman, \{David H.\} and Matthew Fishbaugher and Julie Healer and Cowman, \{Alan F.\} and Kappe, \{Stefan H.I.\} and Manuel Llin{\'a}s",

year = "2013",

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doi = "10.1074/jbc.M113.503557",

language = "English (US)",

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T1 - Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

AU - Cobbold, Simon A.

AU - Vaughan, Ashley M.

AU - Lewis, Ian A.

AU - Painter, Heather J.

AU - Camargo, Nelly

AU - Perlman, David H.

AU - Fishbaugher, Matthew

AU - Healer, Julie

AU - Cowman, Alan F.

AU - Kappe, Stefan H.I.

AU - Llinás, Manuel

PY - 2013/12/20

Y1 - 2013/12/20

N2 - Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.

AB - Background: The acetyl-CoA biosynthetic pathways of the malaria parasite are unclear. Results: 13C-Labeling experiments in parasites lacking a functional pyruvate dehydrogenase (PDH) complex show that thePDH does not contribute significantly to the acetyl-CoA pool. Conclusion: The majority of acetyl-CoA biosynthesis in the parasite derives from a PDH-like enzyme and acetyl-CoA synthetase. Significance: The two routes for acetyl-CoA synthesis appear to have separate functions.

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UR - https://www.scopus.com/pages/publications/84890929025#tab=citedBy

U2 - 10.1074/jbc.M113.503557

DO - 10.1074/jbc.M113.503557

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AN - SCOPUS:84890929025

SN - 0021-9258

VL - 288

SP - 36338

EP - 36350

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 51

ER -

Kinetic flux profiling elucidates two independent acetyl-coa biosynthetic pathways in plasmodium falciparum

Abstract

All Science Journal Classification (ASJC) codes

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