TY - JOUR
T1 - Kinetic study on anaerobic oxidation of methane coupled to denitrification
AU - Yu, Hou
AU - Kashima, Hiroyuki
AU - Regan, John M.
AU - Hussain, Abid
AU - Elbeshbishy, Elsayed
AU - Lee, Hyung Sool
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/9
Y1 - 2017/9
N2 - Monod kinetic parameters provide information required for kinetic analysis of anaerobic oxidation of methane coupled to denitrification (AOM-D). This information is critical for engineering AOM-D processes in wastewater treatment facilities. We first experimentally determined Monod kinetic parameters for an AOM-D enriched culture and obtained the following values: maximum specific growth rate (μmax) 0.121/d, maximum substrate-utilization rate (qmax) 28.8 mmol CH4/g cells-d, half maximum-rate substrate concentration (Ks) 83 μΜ CH4, growth yield (Y) 4.76 g cells/mol CH4, decay coefficient (b) 0.031/d, and threshold substrate concentration (Smin) 28.8 μM CH4. Clone library analysis of 16S rRNA and mcrA gene fragments suggested that AOM-D reactions might have occurred via the syntrophic interaction between denitrifying bacteria (e.g., Ignavibacterium, Acidovorax, and Pseudomonas spp.) and hydrogenotrophic methanogens (Methanobacterium spp.), supporting reverse methanogenesis-dependent AOM-D in our culture. High μmax and qmax, and low Ks for the AOM-D enrichment imply that AOM-D could play a significant role in mitigating atmospheric methane efflux. In addition, these high kinetic features suggest that engineered AOM-D systems may provide a sustainable alternative to nitrogen removal in wastewater treatment.
AB - Monod kinetic parameters provide information required for kinetic analysis of anaerobic oxidation of methane coupled to denitrification (AOM-D). This information is critical for engineering AOM-D processes in wastewater treatment facilities. We first experimentally determined Monod kinetic parameters for an AOM-D enriched culture and obtained the following values: maximum specific growth rate (μmax) 0.121/d, maximum substrate-utilization rate (qmax) 28.8 mmol CH4/g cells-d, half maximum-rate substrate concentration (Ks) 83 μΜ CH4, growth yield (Y) 4.76 g cells/mol CH4, decay coefficient (b) 0.031/d, and threshold substrate concentration (Smin) 28.8 μM CH4. Clone library analysis of 16S rRNA and mcrA gene fragments suggested that AOM-D reactions might have occurred via the syntrophic interaction between denitrifying bacteria (e.g., Ignavibacterium, Acidovorax, and Pseudomonas spp.) and hydrogenotrophic methanogens (Methanobacterium spp.), supporting reverse methanogenesis-dependent AOM-D in our culture. High μmax and qmax, and low Ks for the AOM-D enrichment imply that AOM-D could play a significant role in mitigating atmospheric methane efflux. In addition, these high kinetic features suggest that engineered AOM-D systems may provide a sustainable alternative to nitrogen removal in wastewater treatment.
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U2 - 10.1016/j.enzmictec.2017.05.005
DO - 10.1016/j.enzmictec.2017.05.005
M3 - Article
C2 - 28648180
AN - SCOPUS:85020429319
SN - 0141-0229
VL - 104
SP - 47
EP - 55
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
ER -