TY - JOUR
T1 - Knockin mice with ethanol-insensitive α1-containing γ-aminobutyric acid type A receptors display selective alterations in behavioral responses to ethanol
AU - Werner, David F.
AU - Blednov, Yuri A.
AU - Ariwodola, Olusegun J.
AU - Silberman, Yuval
AU - Logan, Exazevia
AU - Berry, Raymond B.
AU - Borghese, Cecilia M.
AU - Matthews, Douglas B.
AU - Weiner, Jeffrey L.
AU - Harrison, Neil L.
AU - Harris, R. Adron
AU - Homanics, Gregg E.
PY - 2006
Y1 - 2006
N2 - Despite the pervasiveness of alcohol (ethanol) use, it is unclear how the multiple molecular targets for ethanol contribute to its many behavioral effects. The function of GABA type A receptors (GABAA-Rs) is altered by ethanol, but there are multiple subtypes of these receptors, and thus far, individual subunits have not been definitively linked with specific behavioral actions. The α1 subunit of the GABAA-R is the most abundant α subunit in the brain, and the goal of this study was to determine the role of receptors containing this subunit in alcohol action. We designed an α1 subunit with serine 270 to histidine and leucine 277 to alanine mutations that was insensitive to potentiation by ethanol yet retained normal GABA sensitivity and constructed knockin mice containing this mutant subunit. Hippocampal slice recordings from these mice indicated that the mutant receptors were less sensitive to ethanol's potentiating effects. Behaviorally, we observed that mutant mice recovered more quickly from the motor-impairing effects of ethanol and etomidate, but not pentobarbital, and showed increased anxiolytic effects of ethanol. No differences were observed in ethanol-induced hypnosis, locomotor stimulation, cognitive impairment, or in ethanol preference and consumption. Overall, these studies demonstrate that the postsynaptic effects of ethanol at GABAergic synapses containing the α1 subunit are important for specific ethanol-induced behavioral effects.
AB - Despite the pervasiveness of alcohol (ethanol) use, it is unclear how the multiple molecular targets for ethanol contribute to its many behavioral effects. The function of GABA type A receptors (GABAA-Rs) is altered by ethanol, but there are multiple subtypes of these receptors, and thus far, individual subunits have not been definitively linked with specific behavioral actions. The α1 subunit of the GABAA-R is the most abundant α subunit in the brain, and the goal of this study was to determine the role of receptors containing this subunit in alcohol action. We designed an α1 subunit with serine 270 to histidine and leucine 277 to alanine mutations that was insensitive to potentiation by ethanol yet retained normal GABA sensitivity and constructed knockin mice containing this mutant subunit. Hippocampal slice recordings from these mice indicated that the mutant receptors were less sensitive to ethanol's potentiating effects. Behaviorally, we observed that mutant mice recovered more quickly from the motor-impairing effects of ethanol and etomidate, but not pentobarbital, and showed increased anxiolytic effects of ethanol. No differences were observed in ethanol-induced hypnosis, locomotor stimulation, cognitive impairment, or in ethanol preference and consumption. Overall, these studies demonstrate that the postsynaptic effects of ethanol at GABAergic synapses containing the α1 subunit are important for specific ethanol-induced behavioral effects.
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U2 - 10.1124/jpet.106.106161
DO - 10.1124/jpet.106.106161
M3 - Article
C2 - 16785315
AN - SCOPUS:33748990635
SN - 0022-3565
VL - 319
SP - 219
EP - 227
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 1
ER -