Kyotorphin and d-kyotorphin stimulate Met-enkephalin release from rat striatum in vitro

P. K. Janicki; A. W. Lipkowski

doi:10.1016/0304-3940(83)90131-3

Kyotorphin and d-kyotorphin stimulate Met-enkephalin release from rat striatum in vitro

P. K. Janicki
, A. W. Lipkowski

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The release of immunoreactive Met-enkephalin (I-ME) from the rat striatum was studied in vitro using a batch technique. A basal release of the order of 2-3% of total I-ME tissue content per 10 min was found. Both dipeptides kyotorphin and d-kyotorphin produced equipotently dose-dependent I-ME release, with 0.5 mM maximal concentration causing 2-3-fold stimulation oe release. This release was calcium-dependent. In concentrations up to 1 mM both dipeptides did not change the basal release of [³H]noradrenaline, [³H]GABA, [³H]d-aspartate and immunoreactive beta-endorphin from varioys brain structures. The results support a role of kyotorphin as a specific I-ME-releasing factor in the rat brain.

Original language	English (US)
Pages (from-to)	73-77
Number of pages	5
Journal	Neuroscience letters
Volume	43
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(83)90131-3
State	Published - Dec 23 1983

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1016/0304-3940(83)90131-3

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title = "Kyotorphin and d-kyotorphin stimulate Met-enkephalin release from rat striatum in vitro",

abstract = "The release of immunoreactive Met-enkephalin (I-ME) from the rat striatum was studied in vitro using a batch technique. A basal release of the order of 2-3\% of total I-ME tissue content per 10 min was found. Both dipeptides kyotorphin and d-kyotorphin produced equipotently dose-dependent I-ME release, with 0.5 mM maximal concentration causing 2-3-fold stimulation oe release. This release was calcium-dependent. In concentrations up to 1 mM both dipeptides did not change the basal release of [3H]noradrenaline, [3H]GABA, [3H]d-aspartate and immunoreactive beta-endorphin from varioys brain structures. The results support a role of kyotorphin as a specific I-ME-releasing factor in the rat brain.",

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T1 - Kyotorphin and d-kyotorphin stimulate Met-enkephalin release from rat striatum in vitro

AU - Janicki, P. K.

AU - Lipkowski, A. W.

PY - 1983/12/23

Y1 - 1983/12/23

N2 - The release of immunoreactive Met-enkephalin (I-ME) from the rat striatum was studied in vitro using a batch technique. A basal release of the order of 2-3% of total I-ME tissue content per 10 min was found. Both dipeptides kyotorphin and d-kyotorphin produced equipotently dose-dependent I-ME release, with 0.5 mM maximal concentration causing 2-3-fold stimulation oe release. This release was calcium-dependent. In concentrations up to 1 mM both dipeptides did not change the basal release of [3H]noradrenaline, [3H]GABA, [3H]d-aspartate and immunoreactive beta-endorphin from varioys brain structures. The results support a role of kyotorphin as a specific I-ME-releasing factor in the rat brain.

AB - The release of immunoreactive Met-enkephalin (I-ME) from the rat striatum was studied in vitro using a batch technique. A basal release of the order of 2-3% of total I-ME tissue content per 10 min was found. Both dipeptides kyotorphin and d-kyotorphin produced equipotently dose-dependent I-ME release, with 0.5 mM maximal concentration causing 2-3-fold stimulation oe release. This release was calcium-dependent. In concentrations up to 1 mM both dipeptides did not change the basal release of [3H]noradrenaline, [3H]GABA, [3H]d-aspartate and immunoreactive beta-endorphin from varioys brain structures. The results support a role of kyotorphin as a specific I-ME-releasing factor in the rat brain.

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ER -

Kyotorphin and d-kyotorphin stimulate Met-enkephalin release from rat striatum in vitro

Abstract

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