L(-)-10-Formyltetrahydrofolate Is the Cofactor for Glycinamide Ribonucleotide Transformylase from Chicken Liver

Gary K. Smith; P. A. Benkovic; S. J. Benkovic

doi:10.1021/bi00517a013

L(-)-10-Formyltetrahydrofolate Is the Cofactor for Glycinamide Ribonucleotide Transformylase from Chicken Liver

Gary K. Smith
, P. A. Benkovic
, S. J. Benkovic

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

It is shown that l(-)- 10-formyltetrahydrofolate serves as the cofactor for glycinamide ribonucleotide transformylase from chicken liver. The utilization of l(-)-10-formyl-H4folate was not previously recognized, because L-(+)-10-formyl-H4folate is an excellent competitive inhibitor of the enzyme, Ki = 0.75±0.07µM and historically the transformylase assay was carried out with a mixture of diastereomers. The results are discussed in relation to the utilization of L(+)-5, 10-methenyl-H4folate.

Original language	English (US)
Pages (from-to)	4034-4036
Number of pages	3
Journal	Biochemistry
Volume	20
Issue number	14
DOIs	https://doi.org/10.1021/bi00517a013
State	Published - Jul 1981

All Science Journal Classification (ASJC) codes

Biochemistry

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10.1021/bi00517a013

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title = "L(-)-10-Formyltetrahydrofolate Is the Cofactor for Glycinamide Ribonucleotide Transformylase from Chicken Liver",

abstract = "It is shown that l(-)- 10-formyltetrahydrofolate serves as the cofactor for glycinamide ribonucleotide transformylase from chicken liver. The utilization of l(-)-10-formyl-H4folate was not previously recognized, because L-(+)-10-formyl-H4folate is an excellent competitive inhibitor of the enzyme, Ki = 0.75±0.07µM and historically the transformylase assay was carried out with a mixture of diastereomers. The results are discussed in relation to the utilization of L(+)-5, 10-methenyl-H4folate.",

author = "Smith, \{Gary K.\} and Benkovic, \{P. A.\} and Benkovic, \{S. J.\}",

year = "1981",

month = jul,

doi = "10.1021/bi00517a013",

language = "English (US)",

volume = "20",

pages = "4034--4036",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

number = "14",

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T1 - L(-)-10-Formyltetrahydrofolate Is the Cofactor for Glycinamide Ribonucleotide Transformylase from Chicken Liver

AU - Smith, Gary K.

AU - Benkovic, P. A.

AU - Benkovic, S. J.

PY - 1981/7

Y1 - 1981/7

N2 - It is shown that l(-)- 10-formyltetrahydrofolate serves as the cofactor for glycinamide ribonucleotide transformylase from chicken liver. The utilization of l(-)-10-formyl-H4folate was not previously recognized, because L-(+)-10-formyl-H4folate is an excellent competitive inhibitor of the enzyme, Ki = 0.75±0.07µM and historically the transformylase assay was carried out with a mixture of diastereomers. The results are discussed in relation to the utilization of L(+)-5, 10-methenyl-H4folate.

AB - It is shown that l(-)- 10-formyltetrahydrofolate serves as the cofactor for glycinamide ribonucleotide transformylase from chicken liver. The utilization of l(-)-10-formyl-H4folate was not previously recognized, because L-(+)-10-formyl-H4folate is an excellent competitive inhibitor of the enzyme, Ki = 0.75±0.07µM and historically the transformylase assay was carried out with a mixture of diastereomers. The results are discussed in relation to the utilization of L(+)-5, 10-methenyl-H4folate.

UR - https://www.scopus.com/pages/publications/0019874666

UR - https://www.scopus.com/pages/publications/0019874666#tab=citedBy

U2 - 10.1021/bi00517a013

DO - 10.1021/bi00517a013

M3 - Article

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AN - SCOPUS:0019874666

SN - 0006-2960

VL - 20

SP - 4034

EP - 4036

JO - Biochemistry

JF - Biochemistry

IS - 14

ER -

L(-)-10-Formyltetrahydrofolate Is the Cofactor for Glycinamide Ribonucleotide Transformylase from Chicken Liver

Abstract

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