Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip

Muhammad S. Khan; Ketan Dighe; Zhen Wang; Enrique Daza; Aaron S. Schwartz-Duval; Conor P. Rowley; Isaac A. Calvillo; Santosh K. Misra; Leanne T. Labriola; Dipanjan Pan

doi:10.1109/HIC.2017.8227587

Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip

Muhammad S. Khan, Ketan Dighe, Zhen Wang, Enrique Daza, Aaron S. Schwartz-Duval, Conor P. Rowley, Isaac A. Calvillo, Santosh K. Misra, Leanne T. Labriola, Dipanjan Pan

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

10 Scopus citations

Abstract

In this paper, we present a biosensor chip to study two important biomarkers, lactoferrin (LCF) and beta-2-microglubin (B2M) present in our tear fluid. LCF and B2M have direct relevance with dry eye syndrome and diabetic retinopathy in patients respectively. We have developed a multi-layer paper-based biosensor chip connected with handheld printed circuit board (PCB) to monitor the effectiveness of B2M and LCF in contrived tear film (TF) with a wide range of detection (RoD) from 0.001 to 1ng/mL (R²=0.9886) and 1 to 10mg/mL (R²=0.9554) respectively. Multilayer biosensor is composed of graphene nanoplatelet and diblock-co-polymer coated on a filter paper followed by deposition of micro-Au electrodes. Anti-LCF and anti-B2M antibodies were immobilized via 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester (DTSP) as a self-assembled monolayer (SAM) on Au. In addition to electrical measurements in real-time setup, Raman spectral analysis and SEM were utilized to characterize the sensor surface before and after testing the relevant proteins. We anticipate that the fabricated sensor chip has promising application to study dry-eye-disease (DED) and diabetes in Point-of-care (POC) setting.

Original language	English (US)
Title of host publication	2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	72-75
Number of pages	4
ISBN (Electronic)	9781538613924
DOIs	https://doi.org/10.1109/HIC.2017.8227587
State	Published - Dec 19 2017
Event	2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017 - Bethesda, United States Duration: Nov 6 2017 → Nov 8 2017

Publication series

Name	2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
Volume	2017-December

Conference

Conference	2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
Country/Territory	United States
City	Bethesda
Period	11/6/17 → 11/8/17

All Science Journal Classification (ASJC) codes

Health Informatics
Instrumentation
Health(social science)
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/HIC.2017.8227587

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Khan, M. S., Dighe, K., Wang, Z., Daza, E., Schwartz-Duval, A. S., Rowley, C. P., Calvillo, I. A., Misra, S. K., Labriola, L. T., & Pan, D. (2017). Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip. In 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017 (pp. 72-75). (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017; Vol. 2017-December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HIC.2017.8227587

Khan, Muhammad S. ; Dighe, Ketan ; Wang, Zhen et al. / Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip. 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 72-75 (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017).

@inproceedings{3847e6f2c5624508a02244c5562f757f,

title = "Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip",

abstract = "In this paper, we present a biosensor chip to study two important biomarkers, lactoferrin (LCF) and beta-2-microglubin (B2M) present in our tear fluid. LCF and B2M have direct relevance with dry eye syndrome and diabetic retinopathy in patients respectively. We have developed a multi-layer paper-based biosensor chip connected with handheld printed circuit board (PCB) to monitor the effectiveness of B2M and LCF in contrived tear film (TF) with a wide range of detection (RoD) from 0.001 to 1ng/mL (R2=0.9886) and 1 to 10mg/mL (R2=0.9554) respectively. Multilayer biosensor is composed of graphene nanoplatelet and diblock-co-polymer coated on a filter paper followed by deposition of micro-Au electrodes. Anti-LCF and anti-B2M antibodies were immobilized via 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester (DTSP) as a self-assembled monolayer (SAM) on Au. In addition to electrical measurements in real-time setup, Raman spectral analysis and SEM were utilized to characterize the sensor surface before and after testing the relevant proteins. We anticipate that the fabricated sensor chip has promising application to study dry-eye-disease (DED) and diabetes in Point-of-care (POC) setting.",

author = "Khan, {Muhammad S.} and Ketan Dighe and Zhen Wang and Enrique Daza and Schwartz-Duval, {Aaron S.} and Rowley, {Conor P.} and Calvillo, {Isaac A.} and Misra, {Santosh K.} and Labriola, {Leanne T.} and Dipanjan Pan",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017 ; Conference date: 06-11-2017 Through 08-11-2017",

year = "2017",

month = dec,

day = "19",

doi = "10.1109/HIC.2017.8227587",

language = "English (US)",

series = "2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "72--75",

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address = "United States",

}

Khan, MS, Dighe, K, Wang, Z, Daza, E, Schwartz-Duval, AS, Rowley, CP, Calvillo, IA, Misra, SK, Labriola, LT & Pan, D 2017, Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip. in 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017, vol. 2017-December, Institute of Electrical and Electronics Engineers Inc., pp. 72-75, 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017, Bethesda, United States, 11/6/17. https://doi.org/10.1109/HIC.2017.8227587

Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip. / Khan, Muhammad S.; Dighe, Ketan; Wang, Zhen et al.
2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 72-75 (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017; Vol. 2017-December).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip

AU - Khan, Muhammad S.

AU - Dighe, Ketan

AU - Wang, Zhen

AU - Daza, Enrique

AU - Schwartz-Duval, Aaron S.

AU - Rowley, Conor P.

AU - Calvillo, Isaac A.

AU - Misra, Santosh K.

AU - Labriola, Leanne T.

AU - Pan, Dipanjan

PY - 2017/12/19

Y1 - 2017/12/19

N2 - In this paper, we present a biosensor chip to study two important biomarkers, lactoferrin (LCF) and beta-2-microglubin (B2M) present in our tear fluid. LCF and B2M have direct relevance with dry eye syndrome and diabetic retinopathy in patients respectively. We have developed a multi-layer paper-based biosensor chip connected with handheld printed circuit board (PCB) to monitor the effectiveness of B2M and LCF in contrived tear film (TF) with a wide range of detection (RoD) from 0.001 to 1ng/mL (R2=0.9886) and 1 to 10mg/mL (R2=0.9554) respectively. Multilayer biosensor is composed of graphene nanoplatelet and diblock-co-polymer coated on a filter paper followed by deposition of micro-Au electrodes. Anti-LCF and anti-B2M antibodies were immobilized via 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester (DTSP) as a self-assembled monolayer (SAM) on Au. In addition to electrical measurements in real-time setup, Raman spectral analysis and SEM were utilized to characterize the sensor surface before and after testing the relevant proteins. We anticipate that the fabricated sensor chip has promising application to study dry-eye-disease (DED) and diabetes in Point-of-care (POC) setting.

AB - In this paper, we present a biosensor chip to study two important biomarkers, lactoferrin (LCF) and beta-2-microglubin (B2M) present in our tear fluid. LCF and B2M have direct relevance with dry eye syndrome and diabetic retinopathy in patients respectively. We have developed a multi-layer paper-based biosensor chip connected with handheld printed circuit board (PCB) to monitor the effectiveness of B2M and LCF in contrived tear film (TF) with a wide range of detection (RoD) from 0.001 to 1ng/mL (R2=0.9886) and 1 to 10mg/mL (R2=0.9554) respectively. Multilayer biosensor is composed of graphene nanoplatelet and diblock-co-polymer coated on a filter paper followed by deposition of micro-Au electrodes. Anti-LCF and anti-B2M antibodies were immobilized via 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester (DTSP) as a self-assembled monolayer (SAM) on Au. In addition to electrical measurements in real-time setup, Raman spectral analysis and SEM were utilized to characterize the sensor surface before and after testing the relevant proteins. We anticipate that the fabricated sensor chip has promising application to study dry-eye-disease (DED) and diabetes in Point-of-care (POC) setting.

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DO - 10.1109/HIC.2017.8227587

M3 - Conference contribution

AN - SCOPUS:85048573128

T3 - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017

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BT - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017

PB - Institute of Electrical and Electronics Engineers Inc.

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Y2 - 6 November 2017 through 8 November 2017

ER -

Khan MS, Dighe K, Wang Z, Daza E, Schwartz-Duval AS, Rowley CP et al. Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip. In 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 72-75. (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017). doi: 10.1109/HIC.2017.8227587

Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip

Abstract

Publication series

Conference

All Science Journal Classification (ASJC) codes

UN SDGs

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