Lambda red-mediated genetic modification of the insect endosymbiont Sodalis glossinidius

Mauricio H. Pontes; Colin Dale

doi:10.1128/AEM.02166-10

Lambda red-mediated genetic modification of the insect endosymbiont Sodalis glossinidius

Mauricio H. Pontes, Colin Dale

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited paratransgenic insect control strategies.

Original language	English (US)
Pages (from-to)	1918-1920
Number of pages	3
Journal	Applied and environmental microbiology
Volume	77
Issue number	5
DOIs	https://doi.org/10.1128/AEM.02166-10
State	Published - Mar 2011

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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title = "Lambda red-mediated genetic modification of the insect endosymbiont Sodalis glossinidius",

abstract = "In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited paratransgenic insect control strategies.",

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Lambda red-mediated genetic modification of the insect endosymbiont Sodalis glossinidius

Abstract

All Science Journal Classification (ASJC) codes

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