Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP

Andrei V. Karginov; Yan Zou; David Shirvanyants; Pradeep Kota; Nikolay V. Dokholyan; Douglas D. Young; Klaus M. Hahn; Alexander Deiters

doi:10.1021/ja109630v

Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP

Andrei V. Karginov, Yan Zou, David Shirvanyants, Pradeep Kota, Nikolay V. Dokholyan, Douglas D. Young, Klaus M. Hahn, Alexander Deiters

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120 Scopus citations

Abstract

We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.

Original language	English (US)
Pages (from-to)	420-423
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	133
Issue number	3
DOIs	https://doi.org/10.1021/ja109630v
State	Published - Jan 26 2011

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja109630v

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abstract = "We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.",

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AU - Karginov, Andrei V.

AU - Zou, Yan

AU - Shirvanyants, David

AU - Kota, Pradeep

AU - Dokholyan, Nikolay V.

AU - Young, Douglas D.

AU - Hahn, Klaus M.

AU - Deiters, Alexander

PY - 2011/1/26

Y1 - 2011/1/26

N2 - We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.

AB - We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.

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Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP

Abstract

All Science Journal Classification (ASJC) codes

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