TY - JOUR
T1 - Linkage map of mouse chromosome 17
T2 - Localization of 27 new DNA markers
AU - Vincek, Vladimir
AU - Kawaguchi, Hiroshi
AU - Mizuno, Kazuya
AU - Zaleska-Rutczynska, Zofia
AU - Kasahara, Masanori
AU - Forejt, Jiří
AU - Figueroa, Felipe
AU - Klein, Jan
N1 - Funding Information:
We thank all investigatorsme ntioned under Materials and Methods who supplied us with probes or mice. We gratefully acknowledge the excellent technical assistance of Ms. Margarethe Wiirstle and the editorial assistance of Ms. Lynne Yakes. The work was supported in part by the Fonds der Chemischen Industrie, Frankfurt, FRG (Grant 163258) and by Grant 1 R01 AI23667 from the National Institutes of Health. Bethesda, Maryland.
PY - 1989/11
Y1 - 1989/11
N2 - Chromosome 17 of the laboratory variant of the house mouse (Mus musculus L.), MMU17, has been studied extensively, largely because of its involvement in the control of immune response and embryonic as well as male germ cell differentiation. A detailed linkage map of this chromosome is therefore a highly desired goal. As the first step toward achieving this goal, we have isolated, using a LINE 1 repetitive sequence as a probe, 52 anonymous DNA clones from MMU17. Twenty-seven repetitive sequence-free probes isolated from these clones displayed restriction fragment length variation among common inbred strains and could be mapped with the help of recombinant inbred strains, congenic strains, F2 segregants, or intra-t recombinants. Together with markers identified previously, the new markers can be used to construct a map of MMU17 that contains 125 DNA loci. The markers are distributed over a length of approximately 71 cM, which probably represents the entire length of MMU17. Most of the markers reside in the proximal portion of the chromosome, which contains the t and H-2 complexes; this chromosomal region is now fairly well mapped. The distal region of MMU17, on the other hand, is populated by only a few, rather imprecisely mapped markers. Molecular maps are available for most of the H-2 complex and for parts of the t complex.
AB - Chromosome 17 of the laboratory variant of the house mouse (Mus musculus L.), MMU17, has been studied extensively, largely because of its involvement in the control of immune response and embryonic as well as male germ cell differentiation. A detailed linkage map of this chromosome is therefore a highly desired goal. As the first step toward achieving this goal, we have isolated, using a LINE 1 repetitive sequence as a probe, 52 anonymous DNA clones from MMU17. Twenty-seven repetitive sequence-free probes isolated from these clones displayed restriction fragment length variation among common inbred strains and could be mapped with the help of recombinant inbred strains, congenic strains, F2 segregants, or intra-t recombinants. Together with markers identified previously, the new markers can be used to construct a map of MMU17 that contains 125 DNA loci. The markers are distributed over a length of approximately 71 cM, which probably represents the entire length of MMU17. Most of the markers reside in the proximal portion of the chromosome, which contains the t and H-2 complexes; this chromosomal region is now fairly well mapped. The distal region of MMU17, on the other hand, is populated by only a few, rather imprecisely mapped markers. Molecular maps are available for most of the H-2 complex and for parts of the t complex.
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U2 - 10.1016/0888-7543(89)90119-5
DO - 10.1016/0888-7543(89)90119-5
M3 - Article
C2 - 2574145
AN - SCOPUS:0024759520
SN - 0888-7543
VL - 5
SP - 773
EP - 786
JO - Genomics
JF - Genomics
IS - 4
ER -