Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces

Angray S. Kang; Carlos F. Barbas; Kim D. Janda; Stephen J. Benkovic; Richard A. Lerner

doi:10.1073/pnas.88.10.4363

Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces

Angray S. Kang, Carlos F. Barbas, Kim D. Janda, Stephen J. Benkovic, Richard A. Lerner

Chemistry

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401 Scopus citations

Abstract

We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allowing incorporation of functional Fab molecules along the surface of filamentous phage. The power of the method depends upon the linkage of recognition and replication functions and is not limited to antibody molecules.

Original language	English (US)
Pages (from-to)	4363-4366
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	10
DOIs	https://doi.org/10.1073/pnas.88.10.4363
State	Published - May 15 1991

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Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. / Kang, Angray S.; Barbas, Carlos F.; Janda, Kim D. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 10, 15.05.1991, p. 4363-4366.

Research output: Contribution to journal › Article › peer-review

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Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces

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