Lipid rafts: Inhibitors of endocytosis of botulinum neurotoxin

Kimberly A. Petro; Cara Lynne Schengrund

doi:10.1504/TBJ.2010.039112

Lipid rafts: Inhibitors of endocytosis of botulinum neurotoxin

Kimberly A. Petro, Cara Lynne Schengrund

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Methyl-β-cyclodextrin (MβCD) was used to extract cholesterol from plasma membranes of N2a murine neuroblastoma cells. MβCD-disruption of lipid rafts enhanced cleavage of SNAP-25 by BoNT/A, axonogenesis, expression of gangliosides GM1 and GD1a, association of GM1 with the nucleus, and exo- and endo-cytosis. Analysis of endocytosis of an anti-synaptotagmin I monoclonal antibody that recognised part of the protein found on the luminal surface of synaptic vesicles indicated that it was taken up more readily by MβCD-treated cells, supporting the hypothesis that enhanced exocytosis of synaptic vesicles provided more SV2 on the cell surface thereby making more available for toxin binding.

Original language	English (US)
Pages (from-to)	358-374
Number of pages	17
Journal	Botulinum Journal
Volume	1
Issue number	4
DOIs	https://doi.org/10.1504/TBJ.2010.039112
State	Published - 2010

All Science Journal Classification (ASJC) codes

Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Cellular and Molecular Neuroscience

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title = "Lipid rafts: Inhibitors of endocytosis of botulinum neurotoxin",

abstract = "Methyl-β-cyclodextrin (MβCD) was used to extract cholesterol from plasma membranes of N2a murine neuroblastoma cells. MβCD-disruption of lipid rafts enhanced cleavage of SNAP-25 by BoNT/A, axonogenesis, expression of gangliosides GM1 and GD1a, association of GM1 with the nucleus, and exo- and endo-cytosis. Analysis of endocytosis of an anti-synaptotagmin I monoclonal antibody that recognised part of the protein found on the luminal surface of synaptic vesicles indicated that it was taken up more readily by MβCD-treated cells, supporting the hypothesis that enhanced exocytosis of synaptic vesicles provided more SV2 on the cell surface thereby making more available for toxin binding.",

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AU - Petro, Kimberly A.

AU - Schengrund, Cara Lynne

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AB - Methyl-β-cyclodextrin (MβCD) was used to extract cholesterol from plasma membranes of N2a murine neuroblastoma cells. MβCD-disruption of lipid rafts enhanced cleavage of SNAP-25 by BoNT/A, axonogenesis, expression of gangliosides GM1 and GD1a, association of GM1 with the nucleus, and exo- and endo-cytosis. Analysis of endocytosis of an anti-synaptotagmin I monoclonal antibody that recognised part of the protein found on the luminal surface of synaptic vesicles indicated that it was taken up more readily by MβCD-treated cells, supporting the hypothesis that enhanced exocytosis of synaptic vesicles provided more SV2 on the cell surface thereby making more available for toxin binding.

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Lipid rafts: Inhibitors of endocytosis of botulinum neurotoxin

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