Abstract
Methyl-β-cyclodextrin (MβCD) was used to extract cholesterol from plasma membranes of N2a murine neuroblastoma cells. MβCD-disruption of lipid rafts enhanced cleavage of SNAP-25 by BoNT/A, axonogenesis, expression of gangliosides GM1 and GD1a, association of GM1 with the nucleus, and exo- and endo-cytosis. Analysis of endocytosis of an anti-synaptotagmin I monoclonal antibody that recognised part of the protein found on the luminal surface of synaptic vesicles indicated that it was taken up more readily by MβCD-treated cells, supporting the hypothesis that enhanced exocytosis of synaptic vesicles provided more SV2 on the cell surface thereby making more available for toxin binding.
Original language | English (US) |
---|---|
Pages (from-to) | 358-374 |
Number of pages | 17 |
Journal | Botulinum Journal |
Volume | 1 |
Issue number | 4 |
DOIs | |
State | Published - 2010 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Pharmacology, Toxicology and Pharmaceutics(all)
- Cellular and Molecular Neuroscience