@article{ed80cac4b6d54db3a0675503b47b9c3b,
title = "Lipid Synthesis by Rumen Microorgnisms. II. Further Characterization of the Effects of Methionine",
abstract = "Characteristics of the lipids formed by rumen microbes as a result of methionine stimulation were investigated. Tracer studies with acetate, glucose, and long-chain fatty acids revealed substantial transfer of carbon from these sources to complex microbial lipids. The extensive labeling of a variety of lipid compounds, and especially the stimulation of polar lipid formation, suggests that the lipids formed were serving as structural components related to microbial growth. It is believed that most of this new lipid was associated with rumen protozoa.",
author = "Patton, {R. A.} and McCarthy, {R. D.} and Griel, {L. C.}",
note = "Funding Information: Many of the studies on the lipid metabolism of rumen microorganisms have been concerned with the hydrolysis of dietary lipid or the bio-hydrogenation of unsaturated fatty acids originally contained in the ration of the animal (6, 9, 16, 24). Quantitatively significant lipid synthesis by rumen microorganisms has been suggested by Keeney et al. (11) from the analyses of the lipids in tureen microorganisms and milk fat. These authors suggested the probability of synthesis of long-chain fatty acids from rmnen volatile fatty acids. The synthesis of long-chain fatty acids, apparently by the addition of two carbon units to a short-chain fatty acid precursor by pure cultures of rumen bacteria has been demonstrated (1, 27). Whether the final product was a branched, odd, or even carbon number fatty acid depended on the primary precursor (1, 10, 27). The incorporation of these synthesized fatty acids into the structural phospholipids of the cell was likewise shown (10, 27). Bryant and Doetsch (2) and Wegner ]~eceived for publication November 28, 1969. 1 Authorized for publication as paper no. 3701 in the Journal Series of the Pennsylvania Agricultural Experiment Station on November 24, 1969. 2 Supported in part by The Pennsylvania Department of Agriculture and U.S. Public Health Service Grant :HE 03632. a Supported by ]~alston Purina fellowship.",
year = "1970",
doi = "10.3168/jds.S0022-0302(70)86231-2",
language = "English (US)",
volume = "53",
pages = "460--465",
journal = "Journal of dairy science",
issn = "0022-0302",
publisher = "Elsevier Inc.",
number = "4",
}