Lipoplasty technique affects the cellular fate potential of adipose derived stem cells: A comparison of Body Jet and Body Jet with Smart Lipo techniques

The potential of stem cells to replace damaged or malfunctioning tissues has been studied for some time, and recent studies using adipose‐derived mesenchymal stem cells (ADSCs) have shown promise for adult stem cells as a rich, clinically‐relevant population. Our lab has previously determined that the quantity and quality of ADSCs varies depending on patient age and sex, anatomical location of harvest and whether it was a previously treated site with lipoplasty. A clinically‐relevant question is whether method of lipoplasty affects pluripotency of the stem cells. Our lab has shown that harvesting ADSCs using BodyJet and BodyJet with SmartLipo lipoplasty techniques produces a high yield of viable stem cells. Using established plating densities, corrected for stem cell viability measured by flow cytometry, we have reared isolated ADSCs, confirmed by flow cytometry and confocal microscopy, harvested with the BodyJet and BodyJet with SmartLipo techniques. This result suggests that both BodyJet and BodyJet with SmartLipo lipoplasty techniques yield clinically useful ADSC's. Funded, in part, under a grant with the Pennsylvania Department of Health and intramural funds.