TY - JOUR
T1 - Liquid-EM goes viral – visualizing structure and dynamics
AU - Kelly, Deborah F.
AU - DiCecco, Liza Anastasia
AU - Jonaid, G. M.
AU - Dearnaley, William J.
AU - Spilman, Michael S.
AU - Gray, Jennifer L.
AU - Dressel-Dukes, Madeline J.
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/8
Y1 - 2022/8
N2 - Liquid-electron microscopy (EM), the room temperature correlate to cryo-EM, is an exciting new technique delivering real-time data of dynamic reactions in solution. Here, we explain how liquid-EM gained popularity in recent years by examining key experiments conducted on viral assemblies and host–pathogen interactions. We describe developing workflows for specimen preparation, data collection, and computing processes that led to the first high-resolution virus structures in a liquid environment. Equally important, we review why liquid-electron tomography may become the next big thing in biomedical research due to its ability to monitor live viruses entering cells within seconds. Taken together, we pose the idea that liquid-EM can serve as a dynamic complement to current cryo-EM methods, inspiring the “real-time revolution” in nanoscale imaging.
AB - Liquid-electron microscopy (EM), the room temperature correlate to cryo-EM, is an exciting new technique delivering real-time data of dynamic reactions in solution. Here, we explain how liquid-EM gained popularity in recent years by examining key experiments conducted on viral assemblies and host–pathogen interactions. We describe developing workflows for specimen preparation, data collection, and computing processes that led to the first high-resolution virus structures in a liquid environment. Equally important, we review why liquid-electron tomography may become the next big thing in biomedical research due to its ability to monitor live viruses entering cells within seconds. Taken together, we pose the idea that liquid-EM can serve as a dynamic complement to current cryo-EM methods, inspiring the “real-time revolution” in nanoscale imaging.
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U2 - 10.1016/j.sbi.2022.102426
DO - 10.1016/j.sbi.2022.102426
M3 - Article
C2 - 35868163
AN - SCOPUS:85134576611
SN - 0959-440X
VL - 75
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
M1 - 102426
ER -