Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication

Bao An Chau; Venessa Chen; Alan W. Cochrane; Leslie J. Parent; Andrew J. Mouland

doi:10.1016/j.celrep.2022.111968

Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication

Bao An Chau, Venessa Chen, Alan W. Cochrane, Leslie J. Parent, Andrew J. Mouland

Research output: Contribution to journal › Review article › peer-review

17 Scopus citations

Abstract

The leap of retroviruses and coronaviruses from animal hosts to humans has led to two ongoing pandemics and tens of millions of deaths worldwide. Retrovirus and coronavirus nucleocapsid proteins have been studied extensively as potential drug targets due to their central roles in virus replication, among which is their capacity to bind their respective genomic RNAs for packaging into nascent virions. This review focuses on fundamental studies of these nucleocapsid proteins and how their intrinsic abilities to condense through liquid-liquid phase separation (LLPS) contribute to viral replication. Therapeutic targeting of these condensates and methodological advances are also described to address future questions on how phase separation contributes to viral replication.

Original language	English (US)
Article number	111968
Journal	Cell Reports
Volume	42
Issue number	1
DOIs	https://doi.org/10.1016/j.celrep.2022.111968
State	Published - Jan 31 2023

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2022.111968

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title = "Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication",

abstract = "The leap of retroviruses and coronaviruses from animal hosts to humans has led to two ongoing pandemics and tens of millions of deaths worldwide. Retrovirus and coronavirus nucleocapsid proteins have been studied extensively as potential drug targets due to their central roles in virus replication, among which is their capacity to bind their respective genomic RNAs for packaging into nascent virions. This review focuses on fundamental studies of these nucleocapsid proteins and how their intrinsic abilities to condense through liquid-liquid phase separation (LLPS) contribute to viral replication. Therapeutic targeting of these condensates and methodological advances are also described to address future questions on how phase separation contributes to viral replication.",

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AU - Chau, Bao An

AU - Chen, Venessa

AU - Cochrane, Alan W.

AU - Parent, Leslie J.

AU - Mouland, Andrew J.

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AB - The leap of retroviruses and coronaviruses from animal hosts to humans has led to two ongoing pandemics and tens of millions of deaths worldwide. Retrovirus and coronavirus nucleocapsid proteins have been studied extensively as potential drug targets due to their central roles in virus replication, among which is their capacity to bind their respective genomic RNAs for packaging into nascent virions. This review focuses on fundamental studies of these nucleocapsid proteins and how their intrinsic abilities to condense through liquid-liquid phase separation (LLPS) contribute to viral replication. Therapeutic targeting of these condensates and methodological advances are also described to address future questions on how phase separation contributes to viral replication.

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Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication

Abstract

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