Liquid nitrogen preservation of red blood cells for transfusion. A low glycerol - Rapid freeze procedure

Arthur W. Rowe; E. Eyster; A. Kellner

doi:10.1016/S0011-2240(68)80154-3

Liquid nitrogen preservation of red blood cells for transfusion. A low glycerol - Rapid freeze procedure

Arthur W. Rowe, E. Eyster, A. Kellner

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141 Scopus citations

Abstract

A simple and practical method for the cryopreservation of red cells for transfusion has been described. Human red cells suspended in 14% (v/v) glycerol were placed in flat stainless steel containers and frozen by immersion in liquid nitrogen. The frozen blood was stored at -196°C. After thawing, the blood was transferred into plastic blood bags and washed three times, once with mannitol and twice with saline, to remove glycerol and free hemoglobin. Over-all recovery of the cells was greater than 90% and post-transfusion survival of the recovered red cells was comparable to survival of fresh, unfrozen blood. More than 350 units of red cells frozen and thawed by this process have been administered to patients.

Original language	English (US)
Pages (from-to)	119-128
Number of pages	10
Journal	Cryobiology
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/S0011-2240(68)80154-3
State	Published - 1968

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.1016/S0011-2240(68)80154-3

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abstract = "A simple and practical method for the cryopreservation of red cells for transfusion has been described. Human red cells suspended in 14% (v/v) glycerol were placed in flat stainless steel containers and frozen by immersion in liquid nitrogen. The frozen blood was stored at -196°C. After thawing, the blood was transferred into plastic blood bags and washed three times, once with mannitol and twice with saline, to remove glycerol and free hemoglobin. Over-all recovery of the cells was greater than 90% and post-transfusion survival of the recovered red cells was comparable to survival of fresh, unfrozen blood. More than 350 units of red cells frozen and thawed by this process have been administered to patients.",

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AU - Rowe, Arthur W.

AU - Eyster, E.

AU - Kellner, A.

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N2 - A simple and practical method for the cryopreservation of red cells for transfusion has been described. Human red cells suspended in 14% (v/v) glycerol were placed in flat stainless steel containers and frozen by immersion in liquid nitrogen. The frozen blood was stored at -196°C. After thawing, the blood was transferred into plastic blood bags and washed three times, once with mannitol and twice with saline, to remove glycerol and free hemoglobin. Over-all recovery of the cells was greater than 90% and post-transfusion survival of the recovered red cells was comparable to survival of fresh, unfrozen blood. More than 350 units of red cells frozen and thawed by this process have been administered to patients.

AB - A simple and practical method for the cryopreservation of red cells for transfusion has been described. Human red cells suspended in 14% (v/v) glycerol were placed in flat stainless steel containers and frozen by immersion in liquid nitrogen. The frozen blood was stored at -196°C. After thawing, the blood was transferred into plastic blood bags and washed three times, once with mannitol and twice with saline, to remove glycerol and free hemoglobin. Over-all recovery of the cells was greater than 90% and post-transfusion survival of the recovered red cells was comparable to survival of fresh, unfrozen blood. More than 350 units of red cells frozen and thawed by this process have been administered to patients.

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Liquid nitrogen preservation of red blood cells for transfusion. A low glycerol - Rapid freeze procedure

Abstract

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