TY - JOUR
T1 - Localization of common cytotoxic T lymphocyte recognition epitopes on Simian papovavirus sv40 and human papovavirus JC virus T antigens
AU - Deckhut, Alison M.
AU - Tevethia, Mary J.
AU - Haggerty, Sheryl
AU - Frisque, Richard J.
AU - Stevethia, Satvir
N1 - Funding Information:
The studies reported here were supported by Research Grants CA 25000 and CA 27503 to S.S.T., CA 24694 to M.J.T., and CA 38789 to R.J.F. from the National Cancer Institute (Bethesda, MD).
PY - 1991/7
Y1 - 1991/7
N2 - Human papovavirus 1C virus (JCV) and Simian virus 40 (SV40) tumor or T antigens demonstrate considerable sequence homology which is reflected by antibody cross-reactivity. This similarity raised the possibility that JCV and SV40 T antigen also might contain common cytotoxic T lymphocyte (CTL) recognition epitopes. In this study we identified and mapped such sites on the JCV T antigen. C57BI/6 cell lines transformed by JCV/SV40 T antigen chimeras were generated and tested for susceptibility to lysis by five H-2b restricted SV40-specific CTL clones: Y-1, Y-2, Y-3, Y-4, and Y-5. These CTL clones recognize specific epitopes within amino acids 205-219 (site I), 220-233 (sites 11 and III), 369-511 (site IV), and 489-503 (site V) on SV40 T Ag, respectively. The results show that sites I, 11, III, and IV (recognized by CTL clones Y-1, Y-2, Y-3, and Y-4, respectively) represent common epitopes on SV40 and JCV T antigens. CTL clone Y-5 failed to recognize JCV T antigen indicating that CTL can discriminate between the two antigenically related T antigens.
AB - Human papovavirus 1C virus (JCV) and Simian virus 40 (SV40) tumor or T antigens demonstrate considerable sequence homology which is reflected by antibody cross-reactivity. This similarity raised the possibility that JCV and SV40 T antigen also might contain common cytotoxic T lymphocyte (CTL) recognition epitopes. In this study we identified and mapped such sites on the JCV T antigen. C57BI/6 cell lines transformed by JCV/SV40 T antigen chimeras were generated and tested for susceptibility to lysis by five H-2b restricted SV40-specific CTL clones: Y-1, Y-2, Y-3, Y-4, and Y-5. These CTL clones recognize specific epitopes within amino acids 205-219 (site I), 220-233 (sites 11 and III), 369-511 (site IV), and 489-503 (site V) on SV40 T Ag, respectively. The results show that sites I, 11, III, and IV (recognized by CTL clones Y-1, Y-2, Y-3, and Y-4, respectively) represent common epitopes on SV40 and JCV T antigens. CTL clone Y-5 failed to recognize JCV T antigen indicating that CTL can discriminate between the two antigenically related T antigens.
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U2 - 10.1016/0042-6822(91)90125-U
DO - 10.1016/0042-6822(91)90125-U
M3 - Article
C2 - 1711255
AN - SCOPUS:0025863937
SN - 0042-6822
VL - 183
SP - 122
EP - 132
JO - Virology
JF - Virology
IS - 1
ER -